Histopathological Changes in Liver, Kidneys and Testes of Male ICR Mice Fed Annona Muricata Leaves Ethanolic Extract (AMLE)

Meor Yahaya, Maitasha Alia (2021) Histopathological Changes in Liver, Kidneys and Testes of Male ICR Mice Fed Annona Muricata Leaves Ethanolic Extract (AMLE). [Project Paper] (Submitted)

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Abstract

Medicinal plants have been exploited for their therapeutic values throughout human history and the rapid commercialisation of their products has been increasingly observed. Annona muricata is an example of a plant with various medicinal benefits that have been explored throughout past decades particularly for its antiinflammatory, anticancer, and more recently, antifertility properties. Despite this, its toxicity profile is still not well-established. especially in the aspect of histology. In particular, there are limited histopathological profiles on the liver, kidney, and testes if animals were treated with AMLE after one spermatogenic cycle. The significant needs to evaluate the possible damage in selected organs lead to the design of this work. Objective: This study aimed to assess the toxicity effect of feeding Annona muricata leaves ethanol extract (AMLE) for 35 days on the liver, kidneys, and testes of male mice via histopathological examination and evaluation of the changes in relative organ weights (ROW). Methodology: Twenty male ICR mice were divided into four groups; group A acts as control with distilled water whilst treatment groups B, C and D were orally administered with AMLE at dose levels of 100 mg/kg (low dose), 200 mg/kg (medium dose) and 300 mg/kg (high dose) respectively for 35 days. Treatment was terminated on day 35 and mice were euthanised the next day. The liver, kidneys, and testes were harvested and weighed. The organs were subjected to histopathological processes which includes, tissue fixation, tissue processing, tissue embedding, and tissue sectioning to obtain slides that were stained using haematoxylin-eosin (H&E) dye before microscopical examination. Histopathological scorings were given to any histological changes observed. Results: From the ROW evaluation, group D (5.74 ± 0.11) showed statistically significant difference in mean liver weights compared to group A (4.18 ± 0.21). From the histopathological analyses, only two of ten parameters in the treatment groups had significance difference when compared with group A. Discussion: The changes in the ROW and cellular injuries observed at the tissue level might not be pathologically influenced by AMLE consumption. This is supported by the insignificant differences in the histological findings between the treatment groups and control group A. Conclusion: Findings from this study suggest that feeding AMLE for 35 days at the mentioned dosages does not seem to induce toxicity in the liver, kidney, and testes in male mice. However, further toxicity assessment such as chronic studies of AMLE should be conducted to endorse prolonged consumption.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:47
Last Modified:	22 Aug 2023 07:47
URI:	http://psaspb.upm.edu.my/id/eprint/1043

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