Cytotoxic Effect of BHMC on HepG2 Cell Lines via Trypan Blue Exclusion Assay

Mahbud, Nurul Asyikin (2021) Cytotoxic Effect of BHMC on HepG2 Cell Lines via Trypan Blue Exclusion Assay. [Project Paper] (Submitted)

Text FPSK2 2021 22.pdf Download (1MB)

Abstract

Hepatocellular carcinoma (HCC) is one of the primary liver cancers and a leading cause of death due to cancer worldwide. It is often associated with risk factors such as viruses and excessive alcohol consumption. Several bioactive compounds have been explored to target a possible therapeutic approach in cancer treatment and one of it is curcumin. Curcumin, a bioactive compound derived from the rhizomes part of Curcuma longa (turmeric) is known to possess various pharmacological properties in cancer treatment including anti- proliferative, anti- inflammatory and chemotherapeutic. It has been proven that curcumin exhibit anti- cancer effects by inducing cell death and inhibit cell proliferation. Apoptosis is induced by curcumin through exerting direct cytotoxic effect that eventually leads to reduction in cell viability. However, due to its low bioavailability, 2, 6-bis-(4- hydroxyl-3-methoxybenzylidine) cyclohexanone (BHMC), an analogue of curcumin is synthesised to enhance the anti-cancer properties by removing unstable B-diketone moiety from the structure and modify it into conjugated double bond. BHMC is believed to be more selective in terms of cytotoxicity. Objective: This study aims to determine and compare the cytotoxic effect of BHMC and curcumin on the viability of HepG2 and Hs27 cells. Methodology: Percentage of HepG2 and Hs27 cells viability following treatment of BHMC and curcumin were determined by Trypan Blue Exclusion Assay. Results: Treatment with BHMC at 24 hours significantly reduced HepG2 cells at 30-60%. A remarkable reduction at 70-80% of cell populations were observed after 48 hours treatment with lower concentration compared to curcumin. On the contrary, BHMC only reduced 20-30% of Hs27 cells at 24 hours and 40-60% at 48 hours. Meanwhile, curcumin reduced 40-60% of HepG2 cells for 24 and 48 hours but only reduced 30% of Hs27 cells at similar concentration and timepoints. Discussion: Although BHMC and curcumin reduced the percentage of HepG2 cell viability in concentration and time dependent manner, anti-proliferative effect exerted by BHMC was greater compared to curcumin. BHMC also showed a cytotoxic selective effect on normal Hs27 cells especially at 48 hours with less cell death at lower concentration compared to its effect on HepG2 cells. The cyto-selective effect on Hs27 was observed to be similar with its parental compound, curcumin. Conclusion: BHMC mediate greater cytotoxic effect in HepG2 by reducing the cell viability and modulate higher percentage of cell death at lower concentration compared to curcumin. BHMC also possessed a cytotoxic selective effect towards Hs27 cells due to less cell death observed at similar concentrations.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:47
Last Modified:	22 Aug 2023 07:47
URI:	http://psaspb.upm.edu.my/id/eprint/1087

Actions (login required)

View Item