Evaluation of Antioxidant and Anti-psoriatic Activities of Purple Sweet Potato (Ipomea batatas (L.) Lam) Leaves Aqueous Extract on Imiquimod-induced Psoriasis-like Dermatitis

Arjunaidie, Nur Afiqah (2021) Evaluation of Antioxidant and Anti-psoriatic Activities of Purple Sweet Potato (Ipomea batatas (L.) Lam) Leaves Aqueous Extract on Imiquimod-induced Psoriasis-like Dermatitis. [Project Paper] (Submitted)

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Abstract

Psoriasis is a chronic autoimmune skin disease and is one of the most unpredictable and currently incurable and may greatly affect patients’ quality of life. Conventional therapy using corticosteroids, Vitamin D analogs, calcineurin inhibitors and cytotoxic agents is associated with low success rate and their prolonged use may cause severe adverse effects. Thus, the discovery of more effective anti-psoriatic drugs with a minimal side effect is currently an active research area. In Malay traditional medicine, the leaf part of sweet potato (Ipomoea batatas) has been claimed as antipsoriatic. However, scientific reports on its anti-psoriatic activity are very limited. Objective: The aim of this study is to evaluate the antioxidant and anti-psoriatic activities of purple sweet potato leaf aqueous extract (PSPLAE) on imiquimod (IMQ)- induced psoriasis-like dermatitis in Balb/C mice. Methodology: Several antioxidantrelated phytochemical compositions in PSPLAE, namely total phenolic (TPC), total flavonoids (TFC) content, were investigated. In addition, the antioxidant activity of PSPLAE was tested using 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging assay. The PSPLAE was then subjected to Imiquimod, IMQ-induced psoriatic Balb/C mouse model to check the efficacy of PSPLAE against psoriasis-like dermatitis, where treatment was carried out for 15 days topically (PSPLAE cream at 5, 10 and 20%) and their Psoriasis Area Severity Index (PASI) was calculated. Results: From the phytochemical analysis, PSPLAE exhibited the corresponding values of 7.62 ± 1.91mg GAE/g dry extract (DE), and 2.74 ± 0.85 mg QE/g DE for TPC and TFC, respectively. In addition, the antioxidant activity of PSPLAE, which determined by the DPPH assay showed lower activity (EC50 = 244.8 ±13.6 ug/mL) when compared to the ascorbic acid standard (EC50 = 47.3 ±8.9 ug/mL). Interestingly, the antioxidant activity was found positively (r2 = 0.59) correlated with the total phenolic content, TPC. As for the in vivo study, the effect of PSPLAE cream on the IMQ-induced psoriatic mouse model showed a dose-dependent response as evident through the PASI grading. Discussion: The results of the present investigation reveal the anti-psoriatic activity of the aqueous extract of I. batatas leaves against IMQinduced psoriasis-like dermatitis in Balb/C mice. Based on the findings from this study and previous published reports, the mechanism of action of PSPLAE in ameliorating the psoriasis-like dermatitis in the experimental animals is closely related to the antioxidant activity of polyphenolic compounds present in the leaves part of I. batatas. Conclusion: This study suggests that sweet potato leaves are an inexpensive source of natural antioxidants with a possible anti-psoriatic activity.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:15
Last Modified:	22 Aug 2023 07:15
URI:	http://psaspb.upm.edu.my/id/eprint/1121

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