Mechanistic studies of Lawsonia inermis aqueous extracts on ethanol and hydrogen peroxide-induced skin inflammatory conditions

Lee, Yan Qi (2021) Mechanistic studies of Lawsonia inermis aqueous extracts on ethanol and hydrogen peroxide-induced skin inflammatory conditions. [Project Paper] (Submitted)

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Abstract

Common skin inflammatory diseases include psoriasis, atopic dermatitis, acne and cutaneous lupus erythematosus which are characterised by expressions of cytokines and chemokines. Most common treatment modalities for skin inflammatory conditions in present are topical steroids which have some unwanted side effects. Recent studies showed that L. inermis had ability in treating skin inflammatory conditions. The most prominent bioactive compound, lawsone, may involve in anti-inflammatory properties of L. inermis. Objective: This study generally aimed to understand the underlying mechanisms of L. inermis on skin inflammatory conditions. Methodology: A set of data obtained from multiplex analysis involving determination of cytokines expression produced by A431 human skin carcinoma cell lines and 3T3 mouse fibroblast cell lines induced with two kinds of inducers (ethanol and hydrogen peroxide) in acute and chronic conditions were analysed via one-way analysis of variance, followed by Bonferroni post hoc test using IBM SPSS Statistics 25. Inhibitory mechanisms of lawsone on skin inflammatory cytokines (TNF-a & IL-17) were determined via molecular docking method utilizing AutoDock Tools, while the interactions were analysed by PyMOL software and ProteinPlus webserver. Result: For multiplex analysis, in human A431 cell lines, there was no significant difference in TNF-a expression in between L. inermis hot aqueous extract (LIHAE) and L. inermis cold aqueous extract (LICAE) in all concentrations tested. IL-17 and IL-31 were found to be significantly expressed by LIHAE at 23.41ug/ml concentration compared to the negative and positive controls. In mouse 3T3 cell lines, LIHAE and LICAE showed significant expressions of TNF-a compared to the positive and negative controls, and no significant difference in IL-17 expression. For molecular docking analysis, lawsone was able to have strong binding affinity to both TNF-a and IL-17 at -5.0kcal/mol and -6.8kcal/mol respectively. Discussion: Both LIHAE and LICAE showed significant expressions of inflammatory cytokines in A431 and 3T3 cell lines after induction of acute and chronic inflammations. This may due to no to low expression levels of skin inflammatory cytokines in positive and negative controls. For docking analysis, lawsone was predicted to have good inhibitory properties on skin inflammatory cytokines (which are TNF-a and IL-17) as depicted by its strong binding affinities to skin inflammatory cytokines. Conclusion: L. inermis may played an important role in treating skin inflammatory conditions via inhibition of skin inflammatory cytokines (TNF-a, IL-17 & IL-31) but more reliable and thorough studies are needed to support the hypothesis in vitro and in vivo.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:46
Last Modified:	22 Aug 2023 07:46
URI:	http://psaspb.upm.edu.my/id/eprint/1145

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