Shamsu Kamar, Asmah Nurlaili (2021) Cytotoxicity of mitomycin-c loaded on chitosan/mn:zns quantum dots nanocarrier againts non-muscle invasive bladder cancer cells. [Project Paper] (Submitted)
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Abstract
Bladder cancer is the most common genitourinary malignancy, with a majority of the patients was diagnosed with non-muscle invasive bladder cancer (NMIBC). NMIBC is cancer cells found inside the bladder's inner layer, where the bladder muscle is not involved. At present, intravesical mitomycin C (MMC) chemotherapy is a gold standard treatment for NMIBC. However, the drug delivery mechanism and the efficacy of this intravesical procedure are still deficient, resulting in the recurrence of NMIBC. Nevertheless, recent studies have suggested the application of nanoformulation in intravesical chemotherapy to improve drug delivery for NMIBC treatment. Objective: Therefore, this study aims to investigate the cytotoxicity of MMC loaded on chitosan/Mn ZnS quantum dots nanocarrier against NMIBC cells. Methodology: Prior to the MTT assay, different cell densities of RT112 cells (1560 to 50000 cells/well) were seeded in a 96-well plate to determine the optimum cell density. For the cytotoxicity assay, RT112 cells were treated with different concentrations of conventional MMC (non-nanoformulation) (7.8 to 250 uM), nanoformulation MMC (0.78 to 100 uM) and nanocarrier (CS-Mn: ZnS, Mn: ZnS and CS ) (0.07 to 2.08 mg/mL). Following the 24, 48 and 72 hours incubation, the cell viability and the IC50 were calculated. Results: From the cell seeding density optimisation, the cell density of 6250 cells/well was selected for the cytotoxicity assay. Based on the MTT assay, the IC50 values of RT112 cells are 40.5 ± 0.12 uM (24 hours), 2.0 ± 0.27 uM (48 hours) and 0.5 ± 0.55 uM (72 hours). The IC50 values were significantly reduced at 20-fold (48 hours) and 81-fold (72 hours) lower than the IC50 value obtained from 24 hours of incubation, indicates that the conventional MMC is highly cytotoxic towards RT112 cells after a prolonged incubation period. For nanoformulation MMC, a significant (p<0.05) reduction in the cell viability of RT112 cells was observed at the highest concentration (250 uM), following 72 hours of incubation. Unfortunately, no IC50 value was obtained from the present finding, suggesting that the nanoformulation MMC was not effectively killed the bladder cancer cells. A significant (p<0.05) reduction in cell viability of RT112 cells treated with nanocarrier of CS-Mn: ZnS was also observed, suggesting that Mn: ZnS and CS in the nanoformulation probably contribute to the cytotoxicity against RT112 cells. Conclusion: The present study indicates that the nanoformulation MMC did not significantly exhibit cytotoxic properties against NMIBC cells as compared to conventional MMC. Hence, the optimisation of the nanoformulation MMC is highly recommended to improve its efficacy and cytotoxic properties against bladder cancer cells.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms. Nor Safa'aton Saidin |
| Date Deposited: | 22 Aug 2023 07:51 |
| Last Modified: | 22 Aug 2023 07:51 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1148 |
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