The Effects of Novel Pyrazole Derivatives (HEXUR-2) on the Cytotoxicity and Migration of MDA-MB-231 Human Breast Cancer Cells.

Hasran, Siti Sarah (2021) The Effects of Novel Pyrazole Derivatives (HEXUR-2) on the Cytotoxicity and Migration of MDA-MB-231 Human Breast Cancer Cells. [Project Paper] (Submitted)

Text FPSK2 2021 41.pdf Download (3MB)

Abstract

Breast cancer is the most frequent malignant cancer in women and the leading cause of cancer death worldwide. According to GLOBOCAN (2020), the percentage of new cases for women of all ages in Malaysia is 32.9%. Like any other cancer, it possesses the ability to metastasize and invade the surrounding tissues. This ability is often used to distinguish between aggressive and non-aggressive cancers. The treatments for breast cancers are already available, either chemotherapy treatment or removal of the tumor via surgery. In certain cases, both treatments may be required. However, there is still no effective therapy that is capable of killing cancer cells without destroying normal cells. Therefore, in this study, novel pyrazole derivatives (HEXUR-2) will be used to investigate the cytotoxic and migration effects on the MDA-MB-231 Human Breast Cancer Cells. Objectives: The objective of this study is to determine the effects of novel pyrazole derivatives (HEXUR-2) of the cytotoxicity and migration effects on MDA-MB-231 cells. Methodology: This study used MDAMB- 231 cells and were cultured in complete growth medium DMEM under humidified conditions. The compounds used are novel pyrazole derivatives (HEXUR- 2). Cell viability and cytotoxic effects of HEXUR-2 on MDA-MB-231 cells were measured using MTT assay. The migration of MDA-MB-231 cells was observed using scratch migration assay. Results: MTT assay results showed that the IC50 values for MDA-MB-231 cells treated with HEXUR-2 were 75 ± 9.7, 33 ± 9.3 and 9 ± 9.5 μM at 24, 48 and 72 hours of incubation, respectively. The morphological changes such as apoptotic bodies, nuclear fragmentation and cell shrinkage were observed in MDAMB- 231 cells treated with HEXUR-2. Meanwhile, the treatment of HEXUR-2 on the MDA-MB-231 cells migration was observed at 0 and 24 hours. Discussion: HEXUR- 2 was shown to significantly reduced the cytotoxicity effects in a dose-dependent manner with the IC50 observed at three different incubation periods. However, HEXUR-2 did not significantly reduce the cytotoxicity effects of MDA-MB-231 cells in a time-dependent manner. MDA-MB-231 cells treated with HEXUR-2 also showed the apoptotic characteristics such as cell shrinkage, nuclear fragmentation, and cells detachment, suggesting that the mode of the cell death might be due to the apoptosis. The treatment of HEXUR-2 also shown not to inhibit the migration of MDA-MB-231 cells compared to the untreated cells. Conclusion: Therefore, it is concluded that HEXUR-2 exhibits cytotoxic effects however the migration effects were not shown in the MDA-MB-231 cells.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:44
Last Modified:	22 Aug 2023 07:44
URI:	http://psaspb.upm.edu.my/id/eprint/1150

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