Molecular detection of capsular serotypes k1 and k2 klebsiella pneumoniae ESBL Clinicals Isolates

Hazman, Hazmin (2021) Molecular detection of capsular serotypes k1 and k2 klebsiella pneumoniae ESBL Clinicals Isolates. [Project Paper] (Submitted)

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Abstract

Klebsiella pneumoniae is a gram-negative, non-motile encapsulated bacteria which is commonly associated with hospital acquired infections (HAIs). Studies have shown that infections caused by hypervirulent Klebsiella pneumoniae (hvKp) isolates occurred in patients with chronic diseases including asthma, sepsis, pneumonia and liver abscesses. Klebsiella pneumoniae exhibits the highest virulent activity with the presence of K1 and K2 capsule-associated genes, magA and K2A, respectively. Importantly, the treatment of K. pneumoniae infection becomes greatly limited with its ability to produce extended spectrum β - lactamase (ESBL) which causes resistant against almost all β - lactam antibiotics. Objective: The study aims to determine the occurrence of serotypes K1 and K2 in K. pneumoniae ESBL isolates. Methodology: A total of 40 K. pneumoniae ESBL clinical isolates collected from Hospital Pakar Sultanah Fatimah Muar, Johor from 2009 to 2012 were analysed using Multiplex PCR for the presence of magA and K2A genes. Hypermucoviscosity test (String test) was performed to observe the formation of viscous string of K. pneumoniae isolates with more than 5 mm in length. Extraction of DNA was carried out using a commercial extraction kit. Identification of magA and K2A genes were performed using Multiplex PCR followed by DNA sequencing for phylogenetic tree analysis. Result: Five percent (5%) of the isolates were detected as K1 (n = 2) and K2 (n = 2), respectively. High number of the isolates (72.5%) showed positive for the hypermuscoviscosity test. However, hypermuscoviscity test showed no significance associations between K1/K2 and non-K1/K2 capsular serotypes (p = 0.194). The results of phylogenetic analysis of K. pneumoniae K1 and K2 capsular serotypes showed two distinct clusters. Cluster A is represented by K1 while Cluster B is represented by K2 capsular serotypes. Conclusion: High occurrence of non-K1/K2 were observed in this study (90%). Further surveillance of K. pneumoniae isolates from larger number of clinical isolates is important to provide a better understanding and evaluation of the genotypic characteristics and resistance mechanisms of this organism.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:45
Last Modified:	22 Aug 2023 07:45
URI:	http://psaspb.upm.edu.my/id/eprint/1151

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