Determination and comparison of total phenolic, total flavonoid content and antioxidant activity of pineapple wastes (peel and crown

Theng, Wong Yi (2020) Determination and comparison of total phenolic, total flavonoid content and antioxidant activity of pineapple wastes (peel and crown. [Project Paper] (Submitted)

Text FPSK3 2020 10.pdf Download (1MB)

Abstract

The drastic demand of fruits and vegetables increases agricultural crop residues and agro-industrial wastes. Statistically, around 25% to 30% of waste are generated by fruits and vegetables. Pineapple peel and crown are believed to have high phenolic content which has a strong correlation in the antioxidant activity and in turn contributing to cheaper functional food and pharmaceutical products. The objective of this study was to determine and compare the total phenolic content, total flavonoid content and antioxidant activity in peel and crown of pineapple variety MD2. The pineapple peel and crown were collected from Yield Collection Centre under Department of Agriculture Serdang. The samples were ground into small pieces following freeze-dried and extracted using methanol. They were then analyzed using Folin-Ciocalteu assay, aluminium chloride colorimetric method, DPPH assay and FRAP assay. There was no significant difference (p>0.05) between peels (7.182 ± 0.737mg GAE/g dry extract) and crowns (4.878 ± 0.690 mg GAE/g dry extract) of pineapple variety MD2 in total phenolic content. Besides, there was no significant difference (p > 0.05) between peels (2.493 ± 0.139 mg Quercetin/g dry extract) and crowns (2.979 ± 0.274 mg Quercetin/g dry extract) of pineapple variety MD2 in total flavonoid content. The IC50 of pineapple peel (5.924 ± 0.339mg/mL) was significantly lower than that of crown (11.743 ± 0.462 mg/mL). Also, the FRAP value of pineapple peel (2255.207 ± 423.621μM Fe 2+/ g dry extract) was significantly greater (p<0.05) than that of crown (445.896 ± 85.750μM Fe2+/ g dry extract). In addition, total phenolic content was strongly correlated with both DPPH (r=-0.96, p<0.05) and FRAP assay (r=0.97, p<0.05). In brief, pineapple peel is a potential antioxidant while pineapple crown is warranted for further study

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	04 Sep 2023 03:27
Last Modified:	04 Sep 2023 03:27
URI:	http://psaspb.upm.edu.my/id/eprint/1232

Actions (login required)

View Item