Sequence Analysis, 3d Structural Prediction And Epitope Mapping Of LipL41 Protein : Potential Diagnostic Marker For Pathogenic Leptospira

Saudi, Nur Ain Syahirah (2020) Sequence Analysis, 3d Structural Prediction And Epitope Mapping Of LipL41 Protein : Potential Diagnostic Marker For Pathogenic Leptospira. [Project Paper] (Submitted)

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Abstract

Leptospirosis is a zoonotic disease caused by pathogenic spirochete bacteria of the genus Leptospira. Till date, there is a high number of leptospirosis cases reported worldwide and it is highly endemic in Malaysia. The current diagnostics tests that are available have several limitations specifically in early diagnosis of leptospirosis. Lipoprotein L41 (LipL41) is one of the most abundant outer membrane proteins of pathogenic Leptospira species and has been used as a molecular marker in diagnosis of leptospirosis. However, the antigenic diversity among Leptospira spp., is a limiting factor in the diagnosis of the disease. Currently, more than 300 serovars of Leptospira have been identified. Therefore, the study on LipL41 in various strains may help in developing more reliable diagnostic tests in the future. This study aims to identify the consensus sequence of LipL41 in 29 pathogenic strains of seven Leptospira species for protein structure analysis and linear B-cell epitope mapping sequences. Identification of LipL41 mRNA nucleotides and amino acid conserved regions were analysed using bioinformatics tools such as RAST and MUSCLE in JALVIEW viewer. LipL41 secondary and tertiary protein structures were predicted using JPred4 and I-TASSER. The B-cell epitope mapping was performed using BepiPred 2.0 and IEDB 2.0. BLAST analysis revealed that LipL41 only present in pathogenic strains of Leptospira. Multiple sequence alignment showed two LipL41 mRNA and amino acid conserved regions at nucleotide position 1 to 22 and 82 to 100, and 42 to 74 and 143 to 159, respectively, in all the studied strains. Overlapped structure revealed 23% similarity with high coil and helix residual structures were identified among seven Leptospira species. Four conserved B-cell epitopes were identified with three that are predicted to be exposed on the surface of LipL41 protein. The findings suggest that LipL41 mRNA nucleotide, amino acid sequences and B-cell epitope has the potential to be used as an early and accurate diagnostic marker to detect pathogenic Leptospira species, however further studies are needed to support the results.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:56
Last Modified:	22 Aug 2023 07:56
URI:	http://psaspb.upm.edu.my/id/eprint/1286

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