Anti-Inflammatory Effect of Alternanthera sessilis Red (ASR) Aqueous Extract on RAW 264.7 Cells

Mohd Rasid, Muhammad Rusydie (2020) Anti-Inflammatory Effect of Alternanthera sessilis Red (ASR) Aqueous Extract on RAW 264.7 Cells. [Project Paper] (Submitted)

Text FPSK2 2020 13.pdf Download (1MB)

Abstract

Inflammation is a normal and protective physiological response towards foreign body such as injury, infection, infiltration and irritation. Macrophages play an important role in the initiation, maintenance, and resolution of inflammation. Its main functions including antigen presentation, phagocytosis and immunomodulation through production of cytokines and growth factors, thus able to inhibit pathogen replication by releasing a variety of effector molecules, including nitric oxide (NO). However, high level of NO production may induce toxic reactions against other tissue of the host during inflammation particularly in cancer. Thus, NO become a potential molecular therapeutic target for anti-inflammatory therapy. Alternanthera sessilis red (ASR), a cultivar of A. sessilis green has red instead of green aerial parts proved to exhibits anti-inflammatory properties in protein denaturation in vitro. However, the anti-inflammatory mechanism still remains largely unclear. Objective: The aim of this study is to determine the anti-inflammatory effect of ASR aqueous extract on lipopolysaccharides (LPS)-induced RAW 264.7 cells. Methodology: Non-toxic concentration of the ASR was determined by MTT assay on RAW 264.7 cells. In addition, the morphology changes of the cells were observed. Meanwhile, anti-inflammatory effect was determined by measuring NO production from RAW 264.7 cells induced by LPS using Griess reagent. Data was analysed by using one-way analysis of variance (ANOVA) followed by a post hoc Dunnet test. Results and Discussion: MTT assay revealed that only 1000 µg/mLsignificantly (p = 0.018) decreased cell viability. The cells also exhibited no significant changes in its morphology when compared to untreated cells. In NO assay, LPS significantly increased NO production by 3.8-fold compared to untreated group. However, all concentration of ASR significantly (P < 0.001) inhibited NO production induced by LPS. The highest percent of inhibition is exhibited by 500 µg/mL concentration with 71.3%. Conclusion: ASR aqueous extract is found to have no cytotoxic effects and possesses anti-inflammatory effect on RAW 264.7 cells.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:47
Last Modified:	23 Aug 2023 00:47
URI:	http://psaspb.upm.edu.my/id/eprint/1301

Actions (login required)

View Item