Anti-Proliferative Activity of Ardisia crispa on Human Colorectal Cancer Cell; LoVo Cells

Mohd Solha, Ainin Sofiya (2020) Anti-Proliferative Activity of Ardisia crispa on Human Colorectal Cancer Cell; LoVo Cells. [Project Paper] (Submitted)

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Abstract

Cancer is the major disease that kills human worldwide. It is reported that colorectal cancer is the most common cancer type among men and second most common among women in Malaysia. Most of the death caused by the colorectal cancer is at metastatic stage upon diagnosis. At present, colorectal cancer shows great resistance towards chemotherapies treatment which it has been recorded as major limitation. Hence, there are a lot of attempts in effort to stop the progression of this disease by the application of either new synthetic or natural anticancer compound. Ardisia crispa Thunb. A. DC., locally known as “Mata Itik” has been widely used in folklore medicine in treating various inflammatory diseases. Recent studies have reported that Ardisia crispa exhibited various medicinal properties such as anti-inflammatory, anti-oxidative, anti-hyperalgesia and anti-arthritis. Ardisia crispa also has been observed with antiangiogenic properties previously. As metastasis is a part of angiogenesis process, in which angiogenesis is defined as a formation of new blood vessels, therefore, the current study aims to investigate the effect of Ardisia crispa in general and Ardisia crispa root hexane extract (ACRH) in particular on metastatic colorectal cancer cells; known as LoVo cells. This study generally was aimed to evaluate the anti-proliferative activity of ACRH on LoVo cells. In addition, ACRH also was evaluated for its anti-migratory potential. The cytotoxic effect of ACRH has been conducted by previous researcher via MTT assay to evaluate its IC50. Then, scratch assay was conducted on LoVo cells to evaluate the anti-migratory effect of ACRH with three different concentration (2.0, 1.0 and 0.5 µg/mL) for 24 hours. The cytotoxic effect of ACRH was tested via MTT assay where the IC50 was established at 2.1 ± 4.8 µg/mL. However, the anti-migratory effect is not significantly (p>0.05) showed by ACRH treatment when treated with 2.0, 1.0 and 0.5 µg/mL in wound scratch assay. The anti-proliferative potential of ACRH was observed via MTT assay where ACRH was able to show 50% inhibition of cell proliferation when treated with concentration of 2.1 µg/mL on LoVo cells in a concentration-independent manner. Nevertheless, neither ACRH nor sunitinib (positive control) showed any significant inhibition on the cell migration. This might be due to the lower concentrations of ACRH and sunitinib applied in this experiment. From the results obtained, it can be concluded that ACRH has shown potential as anti-proliferative agent. However, it does not exhibit any anti-migratory effect on LoVo cells.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:48
Last Modified:	23 Aug 2023 00:48
URI:	http://psaspb.upm.edu.my/id/eprint/1304

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