Comparison of Antioxidant Activity between Benzimidazole Derivatives NN-1-10 and NN-1-18

Muhammad Fadil Helmi, Nur Hidayah Helmi (2020) Comparison of Antioxidant Activity between Benzimidazole Derivatives NN-1-10 and NN-1-18. [Project Paper] (Submitted)

Text FPSK2 2020 17.pdf Download (1MB)

Abstract

Free radicals are molecules present in every human body that could cause damage to deoxyribonucleic acid (DNA) and proteins and are avoided by antioxidants. Benzimidazole compound and its derivatives have shown antioxidant activities in numerous studies, which further increases their potential as effective synthetic antioxidants. General objective: To evaluate the antioxidant activity of two of the benzimidazole derivatives; NN-1-10 and NN-1-18. Specific objective: To compare the antioxidant capacity of benzimidazole derivatives; NN-1-10 and NN-1-18 using FRAP, DPPH and PAP antioxidant assay. Hypothesis: There is a difference in the antioxidant capacity of benzimidazole derivative NN-1-10 and NN-1-18 in FRAP, DPPH and PAP antioxidant assay. Methodology: The antioxidant properties of NN-1-10 and NN-1-18 were determined through DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay and Polybdenum Antioxidant Power (PAP) assay with concentration of 6.25 – 400 µM and Ferric ion Reducing Antioxidant Power (FRAP) assay with concentration of 3.125 – 200 µM along with controls of each respective assays. Results: For DPPH assay, the EC50 of NN-1-10 = 22.99 ug/ml, while the highest concentration of NN-1-18 (400uM) does not even yields 50% of the standard compound’s DPPH radical scavenging activity. For FRAP assay, the antioxidant activity of NN-1-10 = 141 mmol Fe2+/g while for NN-1-18 = 26.69 mmol Fe2+/g. Conclusion: NN-1-10 shows a higher antioxidant activity than NN-1-18 through FRAP and DPPH assay, which also gives a comparable antioxidant value against the standard compounds.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:49
Last Modified:	23 Aug 2023 00:49
URI:	http://psaspb.upm.edu.my/id/eprint/1305

Actions (login required)

View Item