Ainol Annuar, Nur Farah Nabilah (2020) The Antioxidant Properties of Tocotrienol Rich Fraction (TRF) on Human Bronchial Epithelial Cells (16HBE14o-). [Project Paper] (Submitted)
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Abstract
One of the pathogenesis of asthma is oxidative stress. Oxidative stress occurs when there is an imbalance between free radicals and anti-oxidant in the body system. Free radicals consist of oxygen with uneven number of electrons. Free radicals with uneven number of electrons trigger the chain reaction with other molecules leading to the oxidation process. Oxidative stress due to the increase in production of reactive oxygen species (ROS) leads to chronic inflammation in asthma. Anti-oxidant is capable of donating electrons to the free radicals without unstabilizing themselves, making the free radical less reactive and stable. The bioactive compound that will be administered is an extract of palm oil named tocotrienol rich fraction (TRF) which possesses anti-oxidant effect. Human bronchial epithelial cell line (16HBE14o-) is widely used in research studies to mimic human airway epithelium barrier function. Objective: To investigate the anti-oxidant effect of TRF on human bronchial epithelial cells. Specifically, this project aims to determine the range of non-cytotoxic concentration of TRF on the growth of human bronchial epithelial cell line,namely 16HBE14o-, via cytotoxic assay and to study the morphology of 16HBE14o-. Hypothesis: It is hypothesized that TRF at concentration below 300 ng/mL is not toxic to the growth of 16HBE14o- cells. Methodology: 16HBE14o- was treated with different TRF concentrations (100, 200 and 300 ng/mL) in order to determine the range of non-cytotoxic concentration of TRF on the growth of 16HBE14o- via cytotoxicity assay (MTT). The result obtained was expressed as means ± SEM of 2 independent experiments using GraphPad Prism 8. One-way Analysis of Variance (ANOVA) followed by post hoc comparison by using Dunnett’s test were used to determine the statistical significance (p < 0.05). Results: The normal morphology of 16HBE14o- cells are cobblestone-shaped cells in which the cells have a cuboidal or pebble shape. Based on the statistical analysis, the p-value for all TRF test groups (100, 200 and 300 ng/mL) are not significant when compared to the normal group. All TRF test groups displayed slight elevation in cell viability as compared to normal group. This proves that TRF at the concentrations below 300 ng/mL are non-cytotoxic to the growth of 16HBE14o- cells. Conclusion: TRF was non-cytotoxic on 16HBE14o- cells and TRF may possessed anti-oxidant property at concentrations below 300ng/mL by showing non-cytotoxic effect to the growth of 16HBE14o- cells which is susceptible to allergen.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms. Nor Safa'aton Saidin |
| Date Deposited: | 23 Aug 2023 00:07 |
| Last Modified: | 23 Aug 2023 00:07 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1322 |
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