Molecular typing of hypervirulent genes in Klebsiella pneumoniae recovered from clinical isolates

Rosli, Ainin Farah Hannan (2020) Molecular typing of hypervirulent genes in Klebsiella pneumoniae recovered from clinical isolates. [Project Paper] (Submitted)

Text FPSK2 2020 37.pdf Download (3MB)

Abstract

Klebsiella pneumoniae is a gram-negative, rod-shaped bacterium, non-motile, appears as mucoid and can be found in soil, human’s skin and intestine. Infections due to hypervirulent Klebsiella pneumoniae capsular serotypes K1 and K2 are increasingly reported in patients diagnosed with liver abscess, meningitis, and nosocomial infection. This may be attributed by the presence of polysaccharide capsule, adhesions and aerobactin production. Furthermore, the treatment for K. pneumoniae infection is challenging with its ability to produce extended-spectrum beta-lactamases (ESBLs) which caused resistant against almost all beta-lactam antibiotics except carbapenems. Objective: The current study is conducted to detect the presence of capsular serotypes K1 and K2 hypervirulent genes in K. pneumoniae from the ESBLs clinical isolates. Hypothesis: The strains of K. pneumoniae with ESBL gene isolates from clinical specimen are expected to have hypervirulent genes. Methodology: Ethics clearance is obtained from UPM Medical Research Ethics Committee. A total of 44 K. pneumoniae ESBL producing isolates were obtained from Hospital Pakar Sultanah Fatimah. Hypermucoviscosity test (String test) was carried out to observe the formation of viscous string of K. pneumonia isolates with more than 5mm in length. Extraction of the DNA was done using a commercial extraction kit. Multiplex Polymerase Chain Reaction (PCR) was used to detect the presence of K1 and K2 capsular genes. Visualization of the bands on 1.7% agarose gel for the capsular serotypes K1 and K2 were observed with the presence of 1283bp and 531bp of product size respectively. Results: Biochemical test analysis showed that all the isolates were lactose fermenters and gamma hemolysis. Out of 44 isolates, 35 isolates (79.5%) showed hypermucoviscous phenotype. However, only a small proportion of these isolates were positive for K1 (5%) and for K2 (10%) serotypes detected by Multiplex PCR. Conclusion: 79.5% of positive hypermucoviscosity phenotype does not significantly associated with K1/K2 serotypes. 82% of the ESBL K. pneumoniae clinical isolates were associated with non-K1/K2 serotype. This might be associated to other 76 capsular serotypes.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:53
Last Modified:	23 Aug 2023 00:53
URI:	http://psaspb.upm.edu.my/id/eprint/1327

Actions (login required)

View Item