Antifertility Effects of Annona muricata Leaves Ethanol Extract in Male Mice Reproductive System

Raja Yahya, Raja Muhammad Iqbal (2020) Antifertility Effects of Annona muricata Leaves Ethanol Extract in Male Mice Reproductive System. [Project Paper] (Submitted)

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Abstract

The increase in population is a global concern and regulation of fertility may help to control population growth. Researchers are currently looking at the potential of medicinal plants to address this problem as certain medicinal plants can curb fertility by causing a reduction in reproductive parameters with little or no side-effects. Objective: This study was carried out to determine the anti-fertility effects of Annona muricata leaves ethanol extract (AMLE) on the reproductive system of male mice. Methodology: Twenty sexually mature male mice were divided into four groups; group A (control group) with distilled water while groups B, C and D were given Annona muricata leaves ethanol extract (AMLE) at dose levels of 100 mg/kg (low dose), 200 mg/kg (medium dose) and 300 mg/kg (high dose) respectively. AMLE were administered for 35 days or one spermatogenic cycle. On day 35, all treatment was terminated and 5 mice from each group were selected for libido testing with oestrus females, then left with female mice at a ratio of 1:1 overnight. Subsequently on day 36, the male mice were euthanised and cauda epidydimal sperm were processed for sperm parameters. Mated females were examined for the presence of vaginal plug and uteri were subsequently collected for evidence of implantation sites. Results and Discussion: The results showed that sperm concentration of Groups B, C and D were significantly (p<0.05) reduce at 79.71 ± 1.931, 58.32 ± 4.707 and 41.78 ± 2.925 respectively as compared to control 146.8 ± 2.577. Sperm motility of Groups B, C and D were also significantly (p<0.05) reduced at 70.36 ± 4.079, 57.17 ± 3.194 and 26.73 ±2.731 respectively, as compared to control 91.92 ± 3.592. In addition, sperm viability percentage for Groups B, C and D mice were also significantly (p<0.05) reduced 86.57 ± 2.932, 57.96 ± 1.896 and 42.10 ± 3.264 respectively, as compared to control at 92.93 ± 2.024. Moreover, the significant reduction of sperm concentration, motility and viability were in a dose dependent manner after treatment of AMLE for 35 days. Conclusion: Treatment of Annona muricata leaves ethanol extract (AMLE) fed to male mice for 35 days may have a detrimental effect on sperm parameters by reducing sperm concentration, motility and viability.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:53
Last Modified:	23 Aug 2023 00:53
URI:	http://psaspb.upm.edu.my/id/eprint/1343

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