Comparison Between Death Receptors Expression Level On (H460) Human Lung Cancer Cell Lines And (MRC-5) Normal Lung Cell Line.

Omer, Muhra Romodan (2020) Comparison Between Death Receptors Expression Level On (H460) Human Lung Cancer Cell Lines And (MRC-5) Normal Lung Cell Line. [Project Paper] (Submitted)

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Abstract

Lung cancer is one of the most common cancer types in both developed and developing countries. There are two types of lung cancer; the Non-Small Cell Lung Cancer (NSCLC) and Small Cell Lung Cancer (SCLC). The NSCLC is more frequent and represents about 80% - 85% of lung cancer cases, while the SCLC represents about 10% - 15% of lung cancer cases. Most of the lung cancer related deaths are due to cancer metastasis and late stage diagnoses. Current lung cancer treatments exhibit a considerable number of side effects which necessitate the urgent need for an alternative treatment. One of the propective treatments is the use of Tumour Necrosis Factor-Related Apoptosis Ligand (TRAIL) protein, which effectiveness is highly dependent on the TRAIL cognate surface death receptors 4 and 5 (DR4 and DR5) on NSCLC, but not in normal cells. The level of the receptors on NSCLC has been determined by Fakiruddin et al, (2019), which correlates the effectiveness of TRAIL to the cancer cell. However, the results would need to be further verified using normal lung cell as a control. . Objective: To re-evaluate the findings by Fakiruddin et al., (2019) by comparing the levels of the receptors from NSCLC H460 cells to normal MRC-5 lung cell. Hypothesis: Lung cancer cells express higher level of the death receptors than normal lung cells. Methodology: Two types of lung cells (H460 and MRC-5) were used in this study to compare the expression level of the receptors. NSCLC (H460) and normal lung cell (MRC-5) were cultured and maintained in T75 flask with RPMI medium with supplements necessary for each cell line. When both cell lines reached the targeted confluency ~80%, the cells were harvested into single cell suspension and stained with allophycocyanin (APC)-conjugated TRAIL-R1 (DR4) and TRAIL-R2 (DR5) for flow cytometry analysis of TRAIL agonist (DR4 and DR5). Results: The NSCLC H460 cell line showed higher expression level of DR5 than DR4. However, the normal lung cell (MRC-5) exhibited lower expression of both receptors (DR4 and DR5) when compared to H460. Conclusion: Since the result have shown that the presence of death receptors (DR4 and DR5) in varying proportions in NSCLC is higher than MRC-5 death receptors expression that will facilitate and enable targeting death receptors in cancer stem cell and cancer cells which will cause Apoptosis to the cells but not normal cells.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:52
Last Modified:	23 Aug 2023 00:52
URI:	http://psaspb.upm.edu.my/id/eprint/1344

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