Cytotoxic Effect of Curcumionis Analogue, BHMC on Human Liver Cancer, HepG2 Cell line

Salaebing, Marwah (2022) Cytotoxic Effect of Curcumionis Analogue, BHMC on Human Liver Cancer, HepG2 Cell line. [Project Paper] (Submitted)

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Abstract

Hepatocellular Carcinoma (HCC) is the fourth most common cause of cancer deaths. It is challenging to develop effective HCC therapies due to the chemotherapeutic resistance. Curcumin is a polyphenol compound found in the rhizome of Curcuma longa. It has been linked to several health benefits and it attracted a lot of interest in the scientific and medicine worlds due to its strong anti-angiogenic, anti-inflammatory, antioxidant, and anti-proliferative activities. Several derivatives were synthesized in order to overcome the limitations of curcumin. One of these analogs that syntheses is 2,6-bis-(4-hydroxy-3-methoxybenzylidene) cyclohexanone (BHMC). The synthesis of BHMC was referred to via eliminating the unstable β-diketone moiety and modifying it into a conjugated double bond while retaining phenolic OH functional group. Objective: This study generally aimed to investigate the cytotoxic effect of BHMC and curcumin on human liver cancer, HepG2 cells. Methodology: HepG2 cells were treated with 20, 15, and 10μM concentration of BHMC and 50 and 25μM concentration of curcumin for 24, 48, 72 hours. The morphological changes in the nucleus were detected by Hoechst staining and the cell apoptosis was determined by propidium iodide staining. Results and Discussion: The morphological transformations of HepG2 cells were detected under fluorescence microscopes viewed at magnification of 40x. These morphological changes include DNA fragmentation, formation of apoptotic bodies, condensation of nucleus, and cell shrinkage. BHMC treatment showed Induced the apoptotic cells higher compared curcumin at 24 hours. Conclusion: Nevertheless, this study illustrates that BHMC has a cytotoxic effect against human liver cancer, HepG2 cell lines.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:02
Last Modified:	22 Aug 2023 07:02
URI:	http://psaspb.upm.edu.my/id/eprint/1345

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