The effect of curcuminoid analogue, BHMC on migration and total antioxidant activities in human liver cancer, HepG2 cells

Hanapi, Nur ‘Aqilah ‘Inani (2022) The effect of curcuminoid analogue, BHMC on migration and total antioxidant activities in human liver cancer, HepG2 cells. [Project Paper] (Submitted)

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Abstract

2,6-bis-(4-hydroxyl-3-methoxybenzylidine)cyclohexanone (BHMC) is a derivative of curcumin, a bright yellow natural compound found in the turmeric rhizome, Curcuma longa. Curcumin has been used as a traditional herbal medicine for thousands of years for the treatment of eye infections, wounds, burns, and other skin conditions. The therapeutic actions of curcumin include both anti-oxidant and pro-oxidant, anti-inflammatory, and chemopreventive which prevent proliferation and migration of malignant cells. Reactive Oxygen Species (ROS) are free radicals that interact with oxygen molecules and the accumulation of excessive ROS will cause oxidative stress towards the cells and further lead to inflammation. Curcumin acts as antioxidant as it prevents the accumulation of ROS in normal cells. It also possesses anticancer properties as it has been proven to inhibit cancer progression and suppress cell proliferation and migration through the regulation of enzymes involve in the extracellular remodeling of malignant cells. However, due to its limited bioavailability, BHMC is synthesized to improve these limitations and enhance the therapeutic potential of curcumin. Although not much research done on BHMC, it is proven to possess higher toxicity effect on breast cancer cells when compared to curcumin. Objective: The aim of this study is to determine the effect of BHMC and curcumin on migration and total antioxidant activity in human liver cancer, HepG2 cells. Methodology: The migration of HepG2 cells were observed through scratch assay to determine the percentage of scratch closure after treatment of BHMC and curcumin. Total antioxidant activity in HepG2 cells upon treatment of BHMC and curcumin were determined through FRAP assay. Results and Discussion: The percentage of scratch closure for HepG2 cells treated with BHMC in the measured time point is lower compared to curcumin. Based on biochemical FRAP assay results, BHMC shows significantly lower antioxidant activity as compared to curcumin. However, in cells treated with BHMC or curcumin, FRAP data showed BHMC exerts higher antioxidant levels in time and dose dependent manner compared to untreated. Conclusion: At IC50 value, BHMC exhibits higher effect in reducing the migration rate of HepG2 cells but lower total antioxidant activity on HepG2 cells as compared to curcumin.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 04:41
Last Modified:	22 Aug 2023 04:41
URI:	http://psaspb.upm.edu.my/id/eprint/1354

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