Santhira Sekaran, Tiriishan Reedyy (2022) Neuroprotective effects of Centella asiatica on the expression of apoptotic markers, BCL-2 and BAX in AlCl3 / D- galactose induced Alzheimer’s like male albino Wistar rat model. [Project Paper] (Submitted)
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Abstract
Alzheimer’s disease (AD) is the most common form of dementia with progressive neurodegeneration. Neuronal apoptosis due to the accumulation of amyloid β peptide (Aβ), is a suspected cause of neurodegeneration in AD. There are presently no effective treatment options available for a vast majority of AD patients. Several studies suggest the plant Centella Asiatica (CA) as a potential therapeutic candidate for AD considering its ability to prevent cognitive deterioration and neuron apoptosis by reducing the beta-amyloid toxicity. However, little is known regarding the effects of CA on the expression of mitochondrial apoptotic markers in relation to beta-amyloid toxicity. Objective: This study aims to determine the effects of CA on the expression of mitochondrial anti-apoptotic marker BCL-2 as well as pro-apoptotic marker BAX in D-galactose and aluminium chloride-induced Alzheimer’s like albino Wistar male rat model. Methodology: The rats were randomly distributed into six groups with six rats in each: 1 control group, 1 model group (D-galactose + AlCl3), 1 positive control group (1 mg/kg/ I.p Donepezil), 3 treatment groups (CA100, 200 and 300mg/kg). The dosages of CA were administered along with the model dosage, and the control group received an equivalent volume of distilled water and normal saline for the same period. The rats were euthanized by decapitation, and their brains were excised. ELISA was used to measure the apoptotic markers’ concentration in the brain tissues. Results: Protein expression analyses of rat brains revealed a significant increase in the BCL-2 level and a significant decrease in BAX level in the CA200 and 300 mg/kg group compared to the model group. Discussion: These results show that the extract of CA at 200 and 300 mg/kg can provide neuroprotective effects by inhibiting apoptosis through the increased BCL-2 and reduced BAX expression. However, no significance observed between these two groups indicate that CA200 mg/kg is the most effective dose to reduce apoptosis. Nonetheless, this study is limited in its scope to the sole use of protein quantitation of intrinsic mitochondria-mediated apoptosis proteins, hence additional supplementary research is required to confirm if CA had effect on the markers of interest at these three concentrations. Conclusion: CA can reduce neuronal apoptosis via increased BCL-2 and reduced BAX expression in D-galactose and aluminium chloride-induced Alzheimer’s like albino Wistar male rat model.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms. Nor Safa'aton Saidin |
| Date Deposited: | 22 Aug 2023 07:02 |
| Last Modified: | 22 Aug 2023 07:02 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1370 |
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