Investigating the Effects of Malaysian Tualang Honey (MTH) on Colon Cancer Cells

Mohd Azhari, Nur Khairunnisa (2022) Investigating the Effects of Malaysian Tualang Honey (MTH) on Colon Cancer Cells. [Project Paper] (Submitted)

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Abstract

Colon cancer has been announced as the third most common cancer and the second leading cause of cancer-related death worldwide. Current treatments affect normal cells that lead to varieties of side effects which affect the patients’ life quality. Therefore, exploration of alternatives or adjunct therapy is much needed. The anticancer properties of MTH on cancer cells such as breast and cervical cancer have been well documented. However, gaps in knowledge still exist on the effect of MTH on colon cancer cells. Objectives: This study aimed to investigate the effect of MTH on the proliferation and migration of colon cancer cells, namely HCT-116. Methodology: To obtain the inhibitory concentration (IC50), human colon carcinoma cell line HCT-116 were treated with increasing concentrations of MTH (0.625% - 10%) for 24 hours and the cell viability was assessed using MTT assay. The cells were treated with MTH inhibitory concentrations of 25%, 50% and 75% for 72 hours to determine the proliferation rate via MTT assay. The effect of MTH exposure on the migration of the cells was assessed via migration assay. Results: From the plotted dose-response curve, the IC25, IC50, and IC75 values of HCT-116 cells treated with MTH are 0.9884%, 1.928% and 3.761%. In the proliferation assay, the cell viability of HCT-116 cells decreased significantly as the concentration of MTH increased (p <0.05). The migration assay showed significant reduction in migration rate of HCT-116 cells with exposure to higher MTH concentration (p <0.05). Discussion: Based on the findings, MTH exerted cytotoxic effects on HCT-116 cells in dose-dependent manner, in which consistent with earlier study of MTH on lung adenocarcinoma cells. MTH also inhibited the proliferation of HCT-116 cells. Phenolic content in MTH explained the cytotoxicity and antiproliferation properties of MTH. The study of migration showed migration of HCT-116 cells were suppressed by MTH in concentration-dependent manner. This finding in line with previous study on HCT-116 cells using Manuka honey which proved metastasis of colon cancer were affected by honey. Conclusion: Present study proved that MTH could be a promising potential anticancer agent due its antiproliferative and antimigratory effect on colon cancer cells.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 06:35
Last Modified:	22 Aug 2023 06:35
URI:	http://psaspb.upm.edu.my/id/eprint/1373

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