Association of OmpK36 Porin Variants and the Antimicrobial Resistance Pattern of Klebsiella pneumoniae ESBL and Non-ESBL Producers

Tee, Sze Ni (2022) Association of OmpK36 Porin Variants and the Antimicrobial Resistance Pattern of Klebsiella pneumoniae ESBL and Non-ESBL Producers. [Project Paper] (Submitted)

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Abstract

Klebsiella pneumoniae has caused a wide-spectrum of nosocomial infections and is becoming a significant issue in the health care services worldwide due to its evolving resistance to multiple antimicrobial agents including cephalosporins. This is mainly due to the rapid spread of extended spectrum beta-lactamase (ESBL) strains among K. pneumoniae. Studies have shown that one of the major outer membrane porins of K. pneumoniae known as ompK36 may also play an essential role in the antimicrobial resistance as the channel for the entry of certain antimicrobial agents. Based on ompK36 genotyping, K. pneumoniae isolates can be classified into four major groups (Group A, B, C and D). However, studies on the antimicrobial resistance effects for each variant are still lacking. Objective: The study aims to determine the association of ompK36 porin variants of K. pneumoniae clinical isolates and the antimicrobial resistance patterns of β-lactam antimicrobial agents. Methodology: A total of 163 K. pneumoniae clinical isolates were collected from Hospital Sultanah Aminah Johor Bahru (HSAJB). Antibiotic disc diffusion test was conducted to detect the isolates possessing ESBL-producing phenotype and identify the antimicrobial resistance patterns. DNA of K. pneumoniae were extracted and PCR-based ompK36 typing were carried out to classify the isolates into Group A, B, C and D. Statistical analyses were performed using Chi-square test. Results: In this study, 137 K. pneumoniae isolates were classified into four ompK36 variants, designated groups A (23), B (20), C (60), and D (34), respectively. ESBL-producing K. pneumoniae were shown to express high resistance rates for aztreonam, ceftriaxone, ceftazidime and cefotaxime in comparison to non-ESBL isolates (p<0.001). Group D isolates were shown to poses high resistance rate only against aztreonam (p<0.05). Discussion: Group D is showing higher antimicrobial resistance among all the antimicrobial agents but only proven to have significantly higher antimicrobial resistance against aztreonam. ESBL strains of K. pneumoniae remains its ability to hydrolyse third-generation cephalosporins and aztreonam, resulting in high antimicrobial resistance property. Conclusion: OmpK36 porin variants of ESBL and non-ESBL K. pneumoniae were not associated with the antimicrobial resistance of β-lactam antimicrobial agents.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:04
Last Modified:	22 Aug 2023 07:04
URI:	http://psaspb.upm.edu.my/id/eprint/1375

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