The Effects of Zerumbone on Migration and Invadopodia Formation of Hypoxia-induced Colon Cancer Cells

Mohd Isa, Nurul Iffah (2022) The Effects of Zerumbone on Migration and Invadopodia Formation of Hypoxia-induced Colon Cancer Cells. [Project Paper] (Submitted)

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Abstract

Cancer is a leading cause of death worldwide which metastases is the primary cause of cancer death. Recent treatments that available to treat cancer such as radiotherapy was challenged with the adaptation of cancer cells to the hypoxic condition. In addition, current cancer therapeutic drugs were shown to exert side effects on cancer patients. A natural compound, Zerumbone (ZER) has been widely used in cancer research as several studies proved the effectiveness of ZER in inhibiting cell proliferation and invasion of many cancer cells, including colon cancer cells. Objective: This study aims to investigate the effects of ZER on migration and invadopodia formation of hypoxia-induced HCT116 colon cancer cells. Methodology: Cell viability assay (MTT Assay) was performed to determine the cytotoxic properties of ZER towards HCT116 cell in hypoxic condition. Scratch migration assay and invadopodia assay were conducted to determine the effects of ZER in reducing migration and invadopodia formation of HCT116 cells in the background of hypoxia. Results: Based on MTT result, after HCT116 cells were treated with different concentrations of ZER, it was found that the IC20 values for normoxia were 16.2 µM and 6.8 µM, and 13.0 µM and 5.8 µM in hypoxia at 24- and 48-hours incubation, respectively. IC50 of ZER were determined at 34.9 µM and 13.3 µM in normoxia, and the values were 44.8 µM and 17.0 µM in hypoxia at both incubation time. Treatment of ZER on HCT116 cells migration was observed at 0 and 24 hours while the invadopodia formation of HCT116 cells was observed after reseeding cells on gelatin coated coverslips for 48-hours. Discussion: ZER significantly reduced cell viability in normoxia and hypoxia (p < 0.001). Treatment of ZER at concentration of 35 µM in normoxia, and 44 µM in hypoxia significantly reduced the migration of HCT116 cells (p < 0.001). At concentration of 16 µM and 20 µM for nomoxia and hypoxia, respectively, ZER significantly reduced the invadopodia formation of the cells (p < 0.001). Conclusion: Therefore, it is concluded that ZER shows cytotoxic and inhibitory effects by reducing cell migration and invadopodia formation in colon cancer cells. However, studies on the migration of cells needs to be carried out more.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 06:36
Last Modified:	22 Aug 2023 06:36
URI:	http://psaspb.upm.edu.my/id/eprint/1386

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