Determination of anti-nutrients content before and after heat treatment in selected under utilised leguminous plants in Malaysia

Hassan Nuddin, Nurul Amira (2021) Determination of anti-nutrients content before and after heat treatment in selected under utilised leguminous plants in Malaysia. [Project Paper] (In Press)

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Abstract

Legumes belong to the Fabaceae family of Angiospermae. They are known to be rich in quality proteins and have had a tremendous impact on the nutrition, diet, and health of numerous individuals throughout the world. However, the presence of anti-nutrient such as tannin, saponin and phytate in legumes reduces the bioavailability of minerals and protein digestibility. Therefore, this study was aimed to determine the content of selected anti-nutrients in Pithecellobium jiringa (Jering), Pithecellobium bubalinum (Kerdas), Parkia speciosa (Petai), and Psophocarpus tetragonolobus (Kacang botor) before and after heat treatment. The determination of tannin, phytate and saponin in raw and blanched (blanching for 3 minutes) samples were conducted using the Folin-ciocalteu method, colorimetric assay, and the double extraction gravimetric method, respectively. The data of the study were analysed using one-way ANOVA. The results of raw samples showed that total saponin content ranged between 0.10±0.01% to 0.28±0.00%, total tannin content ranged between 3.81±0.80 to 29.63±2.88 mg TAE/g sample and total phytate content ranged between 0.90±0.24% to 1.16±0.01%. Meanwhile, the results of blanched samples showed that the total saponin content ranged between 0.07±0.05% to 0.21±0.03%, tannin content ranged between 1.07±0.10 to 12.78±1.67 mg TAE/g sample and total phytate content ranged between 0.15±0.13% to 0.47±0.03%. From the finding, heat treatment method (blanching) significantly reduced saponin content in Pithecellobium jiringa and Pithecellobium bubalinum, tannin content in Pithecellobium jiringa and Psophocarpus tetragonolobus and phytate content in all four samples. However, blanching did not significantly reduced the total saponin content in Parkia speciosa, and Psophocarpus tetragonolobus and tannin content in Pithecellobium bubalinum and Parkia speciosa. To conclude, this study shows that most of the anti-nutrient contents in the samples studied were easily reduced by heat treatment. Thus, it is recommended to apply heat treatment when preparing PT, PS, PB and PJ before consumption.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	04 Sep 2023 04:43
Last Modified:	04 Sep 2023 04:43
URI:	http://psaspb.upm.edu.my/id/eprint/1391

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