Cytotoxicity Assessment of 3T3-L1 Diabetic Murine Fibroblast-Treated with Gallic-Acid Loaded Graphene Oxide (GAGO) Nano-Formulation

Syed Kassim, Sharifah Nabilah Huda (2022) Cytotoxicity Assessment of 3T3-L1 Diabetic Murine Fibroblast-Treated with Gallic-Acid Loaded Graphene Oxide (GAGO) Nano-Formulation. [Project Paper] (Submitted)

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Abstract

Diabetes mellitus is one of significant risk factors for chronic wound complications. Thus, the development of Gallic Acid-loaded Graphene Oxide (GAGO) is seen as an alternative treatment for delayed wound especially in diabetic patients. In this study, pharmacological activities of wound healing process. However, the toxicity profile of GAGO nano-formulation in vitro, by far, is only limit on the normal fibroblast cell line. The cytotoxicity of GAGO nano-formulation on diabetic-fibroblast cell line has yet to be assessed. Objective: This study aims to assess the cytotoxicity effects of GAGO on diabetic murine fibroblasts treated cells, with comparison to its native compounds, pure GA and pure GO. Hypothesis: It is hypothesized that the toxicity of GAGO nano-formulation on 3T3-L1-treated cells has been improved than those treated with pure GA or pure GO alone. Methodology: GAGO was exposed to diabetic murine fibroblast (3T3-L1) cells at nine different concentrations ranged between 5μM-500μM. Total viable cell count was performed using MTT assay at 24-, 48-, and 72-hours post-exposure. Results: From the plotted dose-response curve, the IC10 values of 3T3-L1 cells treated with GAGO for 24-, 48- and 72-hour post treatments are 3.298μM, 5.370μM and 2.639μM respectively. Meanwhile the IC20 values are 11.14μM, 28.38μM and 11.83μM for the similar time-points. Treatment of GAGO at concentrations ranging from 0µM to 500µM significantly (p<0.05) reduced cell viability of 3T3-L1 in concentration-dependent manners at three different incubation periods as compared with untreated cells. Discussion: These findings indicate that GAGO significantly increased the viability and exerted less cytotoxic effects of 3T3-L1 cells in time- and concentration-dependent manner compared to its pure GA and pure GO in which consistent with earlier study of GAGO on normal fibroblast cells. Conclusion: Present study suggests that encapsulation of GA on GO nanocarrier improves the toxicity of its pure compounds, GA and GO.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:03
Last Modified:	22 Aug 2023 07:03
URI:	http://psaspb.upm.edu.my/id/eprint/1396

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