Mohd Rashid, Na’imah (2022) Anti-Allergic Inflammatory Effects of Crude Extract and Fractions of Melicope Ptelefolia via Attenuation of IgE-mediated Mast Cell Degranulation. [Project Paper] (Submitted)
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Abstract
Melicope ptelefolia (MP) is a popular medicinal herb commonly consumed to treat a wide range of illnesses including eczema and dermatitis in numerous Asian countries, including Malaysia. An active acetophenone compound, namely 2,4,6-trihydroxy-3-geranylacetophenone (tHGA) found in the leaves of MP, has been previously reported to possess therapeutic effects in various allergic diseases including allergic asthma and atopic dermatitis (AD), attributed to its prominent inhibitory effect against IgE-mediated mast cell degranulation. Therefore, it is hypothesised that the crude extract and fractions of MP may possess anti-allergic inflammatory activities and may have the potential to be produced as nutraceutical products for allergic conditions, especially AD. As mast cells play important role in the pathogenesis of AD, therefore the crude extract and fractions of MP produced by our collaborative team were subjected to in vitro IgE-mediated mast cell degranulation model, to evaluate their mast cell stabilizing activities. Objective: This study aims to evaluate the anti-allergic inflammatory properties of both crude extracts and fractions of MP leaves in rat basophilic leukemia (RBL)-2H3 cells, via IgE-mediated mast cell degranulation assay. Methodology: The crude extract and fractions of MP were assessed for their cytotoxicity at 400, 200, 100, 50, 25 and 12.5 µg/mL via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay in RBL-2H3 cells. Next, dinitrophenyl (DNP)-IgE sensitized RBL-2H3 cells were induced with DNP-bovine serum albumin (BSA) to induce mast cell degranulation, along with several non-cytotoxic concentrations of the extract and fractions. The mast cell stabilizing properties of these extract and fractions were then determined by assessing their inhibitory effects against interleukin (IL)-4 release, a mast cell degranulation marker, during allergic inflammation. Results: Crude extract, XAD-7HP and SP207 fractions were shown to be non-cytotoxic to RBL-2H3 cells up to concentrations of 50, 100 and 200 µg/mL, respectively. In comparison to crude extract and SP207 fraction, a significant reduction in the amount of IL-4 release was observed in cells treated with XAD-7HP fraction extract. Discussion: In comparison to the traditional claims of MP in treating eczema and dermatitis, this study was the first to demonstrate that MP crude extract does not possess mast cell stabilizing activity. UHPLC-MS/MS data produced by our collaborative team revealed that XAD-7HP fraction contained higher flavonoids and acetophenones content compared to SP207 fraction. Since flavonoids have been reported to have antioxidant, anti-inflammation, and anti-allergic properties, the presence of flavonoids is likely to contribute to the mast cell stabilizing activity of XAD-7HP fraction and further enhance the action of acetophenones in inhibiting IL-4 released by DNP-IgE sensitized RBL-2H3. Conclusion: The fraction from the leaves of MP fractionated using XAD-7HP macroporous resin possess better mast cell stabilizing properties than the crude extract and SP207 fraction as a result of higher flavonoids and acetophenones content and may have the potential to be further developed as a nutraceutical for atopic dermatitis.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms. Nor Safa'aton Saidin |
| Date Deposited: | 22 Aug 2023 06:48 |
| Last Modified: | 22 Aug 2023 06:48 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1401 |
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