In-vitro evaluation of the antibacterial activity of red beet peels and date pits on selected bacterial strains

Ahmed, Salma Emad (2021) In-vitro evaluation of the antibacterial activity of red beet peels and date pits on selected bacterial strains. [Project Paper] (Submitted)

Text FPSK3 2021 43.pdf Download (1MB)

Abstract

Microbial contamination poses a global challenge in the food industry. Thus, chemical preservatives are added to extend the shelf life. However, chemical preservatives were found to possess side effects on health when added with exceeded doses or when the containing food is consumed excessively. Red beet peels (RBP) and date pits (DP) are natural by-products rich with quality and quantity bioactive compounds. In this study they were evaluated for their antibacterial activity. By using the sonication bath, different solvent extractions were applied. RBP was extracted with 50% aqueous ethanol + 0.5% acetic acid, and 50% aqueous methanol + 0.1% formic acid. Whereas DP was extracted by 50% aqueous ethanol, methanol, and ethanol. The resultant extracts were administered separately and as an antibiotic synergize (gentamicin) on Salmonella typhimorium, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus using the agar disc diffusion method. There was a significant difference (p<0.05) among certain solvent extractions for both plant samples; however, it was inconsistent with all strains. Generally, levels of susceptibility of the plant extract ranged from weak to moderate due to several possible factors. RBP is suggested to have synergistic activity when combined with the aminoglycoside class of antibiotics compared to the application of the antibiotic only. Aminoglycosides function by inhibiting protein synthesis. Whereas RBP phytochemicals role is suggested to increase cell wall permeability, thus reducing higher antibiotic concentrations. DP is suggested for having antagonistic activity with Escherichia coli, Salmonella tyhpimurium, and Pseudomonas aeruginosa. Antagonism could occur when bacteriostatic drugs reach to the infection site before bactericidal drugs. Another possible factor is the interaction of extracts and antibiotic chemical compounds. In general, more comprehensive methodological approaches are needed in future work to drive an in-depth understanding of plants–antibiotics and plants-microbial mechanisms of action.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	04 Sep 2023 04:28
Last Modified:	04 Sep 2023 04:28
URI:	http://psaspb.upm.edu.my/id/eprint/1424

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