Yasin, Norazlin (2020) Anti-inflammatory activity of Parkia speciosa in rats. [Project Paper] (Submitted)
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Abstract
Parkia speciosa (P. speciosa) or stink beans and locally known as “petai papan” is one of the traditional medicinal plants found in Southeast Asia that contains polyphenol. It is reported that the plant contains high concentration of polyphenol compound and possess anti-inflammatory properties. Currently, there is lack of in vivo study has been conducted to evaluate its potential as an anti-inflammatory substance. Hence, the objectives of this study are to determine the anti-inflammatory activity of P. speciosa extract using three different dosages on lipopolysaccharide (LPS)-induced paw oedema in rats, and to compare the inflammatory activity between the extract and flunixine meglumine in those rats, by evaluating the numbers of total white blood cell (TWBC) and differential WBC, erythron parameters, plasma biochemistry values and also histological scoring of the oedematous paw. Thirty-six rats were divided randomly and equally into six groups. The rat hind paw was injected with LPS (100 µg/100 µL) 30 minutes after the rats were orally administered with the extract at three different concentrations (100 mg/kg, 200mg/kg, 400mg/kg). The groups administered with the extract were named as groups I to III, group IV was flunixine meglumine (2.5 mg/kg) group which served as the positive control, group V acted as the negative control and group VI as control. Paw oedema thickness, total WBC number and its differential, erythron parameters, plasma biochemistry and histology of the paw were evaluated. Results showed significant reduction (p < 0.05) in the paw oedema thickness at all concentrations of P. speciosa extract (100, 200 and 400 mg/kg) starting at 5 hours after LPS was infected, and the results were comparable with flunixine meglumine. Significant reduction in the ratio of neutrophils to lymphocytes was observed in rats given 200 mg/kg P. speciosa extract at the end of the experiment compared to the other groups. Histological scoring of the inflammatory reaction and oedematous lesion in the paws showed significant reduction (p < 0.05) in rats treated with three concentrations of P. speciosa extract compared to negative control group. The findings conclude P. speciosa extract possesses anti-stress and anti-inflammatory activities in rats.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms Nurhaznita Mahmood |
| Date Deposited: | 19 Oct 2023 04:25 |
| Last Modified: | 19 Oct 2023 04:25 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1476 |
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