Mohamad Zamri, Nor Amirah (2020) Effect of immersion fixatives on histological features of red tilapia (Oreochromis spp.) gill, skin and muscle tissues. [Project Paper] (Submitted)
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Abstract
There are increasing interests in histopathological investigation of fishes nowadays. Fixation is one of the important steps in the histology process and an ideal fixative is a fixative that can preserve morphology as close to the living state as possible. A decalcification treatment is crucial to remove calcium phosphate from the calcified tissue to make the tissue soft enough to be sectioning. Up until now, there is still a lack of information about the usage of suitable fixatives and decalcifiers that produce good histological micrograph in tilapia fish tissue. Thus, in this study, we aim to determine the most suitable fixatives and decalcification treatment specifically to fix the gill, skin and muscle tissues of the Red Tilapia (Oreochromis spp.). In this study, four types of fixatives namely 10% Buffered Formalin, 20% Buffered Formalin, Bouin’s solution Davidson’s solution and 2 types of decalcifiers which are formic acid with 10% formalin and formic acid with sodium citrate were used. The abovementioned tissues were collected from five fish and put in the different fixatives before processed accordingly and stained with routine staining for histological observation. Five regions of interests were randomly selected and scored on these parameters; overall morphological clarity of nuclei, staining intensity, the integrity of other tissues, lifting of the cartilage, space formation and the ease to section a specimen. The scoring data was collected and the statistical analysis was done. Based on the results, Bouin’s solution has the highest total mean score of all parameters for gills sample, but the 10% Formalin and 20% Formalin solution are also the suitable fixatives that used for gill. Meanwhile, for skin and muscle tissues, 20% formalin recorded the highest total mean score, but the 10% Formalin and Bouin’s solution also can be used for skin, while for muscle, Bouin’s solution also suitable to use as fixatives. For the best decalcification treatment, the most suitable decalcifiers used is buffered formic acid for 10 hours based on the ease to section a specimen parameter evaluated. In conclusion, different fixatives will yield different results in a different type of tissues. However, other factors should be considered such as duration of fixation, the temperature of the fixative and also its pH for future works.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms. Nordeena Abdul Aziz |
| Date Deposited: | 23 Oct 2023 03:23 |
| Last Modified: | 23 Oct 2023 03:23 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1484 |
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