Serological and molecular detection of west nile virus in Rattus Sp. in Klang Valley

Murugasu, Tharshaini (2020) Serological and molecular detection of west nile virus in Rattus Sp. in Klang Valley. [Project Paper] (Submitted)

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Abstract

West Nile virus (WNV) is a mosquito borne RNA virus belonging to the genus of Flavivirus. It is transmitted via an enzootic cycle involving wild birds, mosquitoes and dead end hosts such as mammals, reptiles and amphibians. The amplifying reservoirs hosts are birds while the primary vector is Culex sp mosquitoes. Studies conducted in Malaysia revealed that WNV antibodies and/or antigen were detected in indigenous people, bats, captive birds, migratory wild birds, macaque, horses and cattle. However, there is no prominent evidence on the WNV prevalence in wild rodents as they are considered reservoirs for most of the viruses worldwide. Our study was mainly aiming to detect WNV by both serological and molecular approach in Rattus sp. Twenty three archived blood serum samples collected from captured rats in Klang Valley wet market area, Malaysia were subjected to serological and molecular assay using commercial IgG c-ELISA kit and one-step RT-PCR, respectively targeting WNV pre-membrane and capsid protein . None of the wild rodent serum samples were positive for IgG c-ELISA and RT-PCR. In conclusion, the rats found in the area might be were not exposed to WNV or having a scarce viraemic level that cannot be detected.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	19 Oct 2023 08:04
Last Modified:	19 Oct 2023 08:04
URI:	http://psaspb.upm.edu.my/id/eprint/1494

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