Molecular detection and genotyping of hepatozoon (apicomplexa: hepatozoidae) infecting peri-domestic rodents in Selangor, Malaysia

Mohd Nasir., Muhammad Syahir Najmi (2020) Molecular detection and genotyping of hepatozoon (apicomplexa: hepatozoidae) infecting peri-domestic rodents in Selangor, Malaysia. [Project Paper] (Submitted)

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Abstract

The diversity of pathogens harbored by rodents have been the focus of many studies due to their potential as vectors of zoonotic diseases. However, there remains a paucity of information on the molecular detection of haemoparasites among these small mammals in Malaysia. The present study was undertaken to determine the molecular prevalence, species diversity and genetic variability of rodent-borne Hepatozoon in the country. A total of 58 peri-domestic rodents were obtained using mesh wire traps form five commercial and residential localities in Selangor, Malaysia. Of the rodents trapped, 24% were from residential areas, while 76% were from commercial areas. Blood was collected for microscopy and molecular detection of Hepatozoon. Following genomic DNA extraction, a partial fragment of the Hepatozoon 18SSUrRNA gene was amplified using genus-specific primers. Microscopy examination of thin blood films revealed a prevalence of 17%, while Hepatozoon DNA was detected in 25.9% of the rodents examined. The prevalence of Hepatozoon among the rodents trapped from the commercial and residential areas were 22.4% and 3.5%, respectively. Bioinformatic and phylogenetic analyses of the 18SSUrRNA gene locus of 15 positive amplicons using Maximum Parsimony (MP) and Neighbor-joining (NJ) algorithms revealed distinct clustering of the Malaysian isolates, with closest affinities to Hepatozoon sp. isolates detected in Chile (Rattus norvegicus), Thailand (Bandicote indica and Amblyomma varanense). The present study constitutes the first report, molecular detection, and genotyping of Hepatozoon among rodents in Malaysia.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	09 Nov 2023 03:30
Last Modified:	09 Nov 2023 03:30
URI:	http://psaspb.upm.edu.my/id/eprint/1522

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