Synergistic effect of garlic and clove oil with antibiotics against Staphylococcus aureus

Hashim, Abdullah Ehsan (2020) Synergistic effect of garlic and clove oil with antibiotics against Staphylococcus aureus. [Project Paper] (Submitted)

Text lp FPV 2020 106.pdf Download (1MB)

Abstract

Combination therapy has been used in the recent years among antibiotics, but the use of combination of antibiotic with medicinal plants are yet to be seen. The combination therapy between antibiotic and medicinal plants has great potential. The aim of the current study was to determine the antimicrobial potential and synergistic effect of garlic and clove oil when combined with common antibiotics against Staphylococcus aureus. The plant extracts were screened for their antimicrobial activity using disc diffusion method. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) were assessed using broth microdilution method. Both plant extracts exhibited antimicrobial activity to S. aureus. The MIC value of garlic extract was 30% while MBC value for garlic extract was 65%; and the MBC value for clove oil extract was 75%. The synergistic effects of garlic extract were observed in the combination with neomycin and gentamicin. The inhibition zones were significantly increased in combination of garlic extract with neomycin (P<0.05) and gentamicin (P<0.05). There was no significant increase of inhibition zone between combination of clove extract with all antibiotics tested, and also with the combination of garlic extract with penicillin G. The percentage increase of inhibition zones between combination of garlic extract with neomycin and gentamicin were 104% and 55%, respectively. In conclusion, there is high antimicrobial potential of both plant extracts and only garlic extract had synergistic effect with certain antibiotics tested. The synergistic effect from the combination of garlic extract and antibiotics may offer alternative of combination therapy against S. aureus infection.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	09 Nov 2023 03:46
Last Modified:	09 Nov 2023 03:46
URI:	http://psaspb.upm.edu.my/id/eprint/1531

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