The usage of effective microorganism in litter management in a broiler farm

Rahmat, Nur Aqilah (2020) The usage of effective microorganism in litter management in a broiler farm. [Project Paper] (Submitted)

Text lp FPV 2020 124.pdf Download (1MB)

Abstract

The poultry industry is known as one of the largest and fastest-growing of agro-based industries in the world. Due to constantly increasing demand for chicken products, problems related to chicken manure or litter management becoming a major concern among the farmers in many Asian countries. An effective microorganism (EM) which consists of a high population of lactic acid bacteria such as Lactobacillus spp. is effective as a probiotic, waste treatment and biological control agent. The application of EM in litter management help in reducing the ammonia level and also inoculate the litter with beneficial microorganisms that will compete for nutrients against the pathogenic ones. Thus, this study aims to determine the viability of the EM post-application in the environment and to evaluate the effectiveness of the sawdust treated with EM. This study was conducted in a poultry farm in Selangor where they practiced applying the EM spray once every three days on the sawdust. In the first experiment, three different EM dilutions (1 :20, 1 :40 and 1 :60) were mixed and left in the environment for up to 72 hours. Samples of each dilution were taken at O h and after 72 h for bacterial culture. MRS broth supplemented with 3 % calcium carbonate was used as preparing media for the bacterial identification where specifically the lactic acid bacteria were observed as clear zone formed around the colony. In the second experiment, sawdust samples were taken from the chicken house at O before the EM application, and at 6, 12, 24, 3 6,48, 72 hrs after EM spray applications. The samples then were mixed with peptone water with the dilution of 1 :9 followed by l 0-fold serial dilutions and bacterial culture done by using the spread plate method on MacConkey agar. The plates were incubated overnight and total plate count was recorded by using colony counter. The results showed that the total number of colony count tend to decrease in time after EM application on the sawdust from Oto 24 h post- application. A sudden increase was recorded at 3 6 and 48 h and dropped again at 72 h. The survival of the lactic acid bacteria and the zoonotic pathogens also measured in this study and identified as Escherichia coli, Klebsiella pneumoniae, Staphylococcus intermedius, and Staphylococcus spp. In conclusion, EM helps against the pathogens which reside in the manure and the application of the EM spray is suggested to be revised to every 2 days. It is recommended to identify the bacterial species in the EM solution and compare the colony density before and after EM spray application for future works.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	09 Nov 2023 08:48
Last Modified:	09 Nov 2023 08:48
URI:	http://psaspb.upm.edu.my/id/eprint/1532

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