Embryonic development study on the induced breeding of hyrbid catfish (Clarias microstomus X Clarias gariepinus) & enrichment of Artemia franciscana with synbiotic

Vincent Mojilis, Miguel Isaiah (2023) Embryonic development study on the induced breeding of hyrbid catfish (Clarias microstomus X Clarias gariepinus) & enrichment of Artemia franciscana with synbiotic. [Project Paper] (Submitted)

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Abstract

Hybridization is the mating or crossover of two different species, and the resulting offspring are referred to as hybrids. Induced breeding using Ovaprim (USA) hormones of standard formula as 0.5 mL/kg for female and 0.25 mL/kg for male of the Clarias gariepinus and Clarias microstomus were successful and the embryonic development of the cross hybrid were studied. The embryonic development larvae were found to go through all the major stages similar to as described for other Clariidae species such as the Giant African Catfish, Heterobranchus bidorsalis as reported by Hilaire et al., (2014). The Morula and Blastula stage occurred at the 4th and 5th hour after fertilization, respectively. The fertilization rate, hatching rate, and survival rate during the first 72 hours for the hybrid crosses were 85.25%, 58.63%and 85.76%. Artemia enrichment was also studied using synbiotics of 4 different treatment (T1: L4AM2 (Enterococcus faecium + inulin), T2: L1M10 (Enterococcus faecalis + inulin), T3: L4AM5 (Enterococcus faecium + inulin) and T4: control. Sampling was done every 2, 4 and 6 hour through dilution sampling. T1 of 108 CFU/ml Enterococcus faecium + 5g/L inulin had the highest bacterial count in the 2nd and 6th hour.

Item Type:	Project Paper
Faculty:	Faculty of Agriculture and Forestry Sciences
Depositing User:	Mr. Azman Mohamad
Date Deposited:	12 Mar 2024 00:10
Last Modified:	12 Mar 2024 00:10
URI:	http://psaspb.upm.edu.my/id/eprint/1647

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