Immunogenicity of genotype-matched Newcastle disease virus vaccine following single eyedrop vaccination in specific-pathogen-free chickens

Ong, Yu Yen (2022) Immunogenicity of genotype-matched Newcastle disease virus vaccine following single eyedrop vaccination in specific-pathogen-free chickens. [Project Paper] (Submitted)

Text lp FPV 2022 15.pdf Download (1MB)

Abstract

Newcastle disease (ND) outbreaks are still being reported despite intensive vaccination programme, causing substantial losses worldwide. Most commercial ND vaccines belong to genotype I or II, while the field strain responsible for outbreaks is genotype VII. A genotype-matched ND vaccine, mIBS025 has been developed recently. This study aimed to investigate the immunogenicity of mIBS025 following single eyedrop (ED) vaccination with different doses of vaccine virus in specific-pathogen-free (SPF) chickens. 75 SPF chickens of 14-day-old were divided equally into five groups. Group 1 was administered with phosphate-buffered saline as unvaccinated control group. Group 2 to 5 were vaccinated with mIBS025 through ED with 104.5, 105, 105.5, and 106 embryo infectious dose 50 (EID50), respectively. Blood samples were collected from all birds at 7, 14, and 21 days post-vaccination (dpv) to determine ND antibody titers through haemagglutination inhibition test. All birds were monitored daily for post-vaccination clinical signs for three weeks. At 7 dpv, antibody titers of 105.5 EID50 group is significantly (p < 0.05) higher (6.73 log2) than 104.5 EID50 (5.50 log2). At 21 dpv, antibody titers of 106 EID50 group is significantly (p < 0.05) higher (9.09 log2) than 105 EID50 (8.12 log2). All birds did not exhibit post-vaccination side effects. 104.5 EID50 was regarded as the optimal dose of mIBS025 as it has induced protective titers (≥ 5 log2) against genotype VII ND virus at 7 dpv. Therefore, dose with a lower requirement of vaccine virus was chosen. This study provides information on immunogenicity of mIBS025 for future study.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	19 Mar 2024 07:01
Last Modified:	19 Mar 2024 07:01
URI:	http://psaspb.upm.edu.my/id/eprint/1672

Actions (login required)

View Item