Histological assessment of epithelial-mesenchymal transition in polycystic kidneys in tigers (Panthera tigris)

Moisol @ Maisol, Hennsolee (2022) Histological assessment of epithelial-mesenchymal transition in polycystic kidneys in tigers (Panthera tigris). [Project Paper] (Submitted)

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Abstract

Polycystic kidney disease (PKD) is an inherited disease known that results in the development of fluid-filled cysts in the kidneys. This is associated with a process known as epithelial-mesenchymal transition (EMT). Despite being reported in humans and domestic cats like the Persian breed, this condition is not well-reported in wild felids. This study describes and evaluates the histological changes of kidneys of tigers (Panthera tigris) using routine, special histochemical, and immunohistochemical stains to understand the role of EMT in the pathophysiology of PKD. Archived samples of kidneys from PKD tiger and normal tiger were used in this study. They were routinely processed, sectioned, and stained with haematoxylin and eosin (HE), Masson’s trichrome, Vimentin, E-cadherin, and β-catenin. These slides were viewed and were assessed for their severity score using the ImageJ software. The kidneys of PKD tigers showed several histopathological lesions like multiple cysts with various sizes lined by thinned epithelium, pale eosinophilic with slightly vacuolated cytoplasm and indistinct demarcation between the tubules. The histopathological change severity score in the renal cortex and medulla of PKD tiger was (4 �} 0.31) and (4 �} 0.47), respectively, which were significantly (p<0.05) higher than the control kidneys that displayed no lesion in both cortex and medulla. For Masson’s trichrome and all three immunohistochemical markers, the distribution ranging between 50-120 pixels in renal cortex and medulla of PKD tigers, significantly (p<0.05) higher compared to the control renal cortex and medulla that showed distribution ranging between 5-40 pixels. In conclusion, development of cysts in the kidneys of PKD tigers involved significant expression of mesenchymal markers by the renal epithelia and stroma.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	25 Mar 2024 03:25
Last Modified:	25 Mar 2024 03:25
URI:	http://psaspb.upm.edu.my/id/eprint/1702

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