Occurrence of pathogenic Vibrio spp. in edible sea molluscs species in Pulau Aman, Pulau Pinang.

Ahmad Sabri, Nurul Husna (2022) Occurrence of pathogenic Vibrio spp. in edible sea molluscs species in Pulau Aman, Pulau Pinang. [Project Paper] (Submitted)

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Abstract

Vibrio spp. consist of potentially pathogenic bacteria that may lead to gastroenteritis in humans through the consumption of raw or undercooked contaminated shellfish. Sea molluscs such as gastropods and bivalves may harbour pathogenic bacteria through their filter-feeding behaviour from the surrounding environment. This study aimed to identify the presence of pathogenic Vibrio spp. in sea molluscs and to determine the susceptibility of the obtained Vibrio spp. against commonly used antibiotics. In this study, three main edible sea molluscs species were collected from Pulau Aman, Pulau Pinang. A total of 25 pooled samples were selected from Siput Gayam (Callista chinensis), Siput Biji Nangka (Glauconome virens) and Siput Unam (Volegalea cochlidium) . The samples were subjected for isolation of Vibrio spp. on selective Thiosulfate-Citrate-Bile Salt-Sucrose (TCBS) agar and identification using Analytical Profile Index (API) 20E Test. All presumptive isolates were found to be Gram negative and were further identified as Vibrio alginolyticus (49.02%), Vibrio parahaemolyticus (49.02%) and Vibrio vulnificus (1.96%). Both Vibrio alginolyticus and Vibrio parahaemolyticus were present in all samples while Vibrio vulnificus was found only in 10% of Siput Biji Nangka. All isolates were further tested for their antibiotic sensitivity using standard disc diffusion methods. It was observed that 100% of the isolates were susceptible to chloramphenicol, oxytetracycline and florfenicol, followed by trimethoprim (41.67%). However, all of the isolates were resistant towards ampicillin and amoxicillin (100%). Results revealed that pathogenic Vibrio spp. are present in these sea molluscs and the bacteria were resistant to some of the tested antibiotics. Thus, if the bacteria survive from inadequate preparation or cooking, consuming these sea molluscs may pose a risk to the public health.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	26 Mar 2024 03:38
Last Modified:	26 Mar 2024 03:38
URI:	http://psaspb.upm.edu.my/id/eprint/1703

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