Effect of different concentration of red palm oil in tris-based extender on the bull’s sperm progressive motility

Mohd Fadhli, Farah Naqiyah (2022) Effect of different concentration of red palm oil in tris-based extender on the bull’s sperm progressive motility. [Project Paper] (Submitted)

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Abstract

Tris-based extenders are widely used in preserving semen for selective breeding. The technique used to store the semen is called cryopreservation where the extended semen is kept in liquid nitrogen. However, this technique causes damage to the sperm cells. Red palm oil (RPO) is an antioxidant rich in carotenoids and vitamin E which may help prevent cellular damage. This study aims to evaluate the effects of different concentrations of RPO in Tris extender on the sperm progressive motility of chilled and frozen-thawed bull semen. Two semen samples were collected from two bulls via electroejaculation. The semen samples were evaluated and diluted in a Tris-based extender with different concentrations of RPO, 0% (negative control), 4%, 8%, 12%, 16%, 20%. The RPO was emulsified using a nano-emulsion procedure. First, the solutions were mixed using a coarse homogenizer before adding into a high-pressure homogenizer to produce nanosized particles which will improve in the mixing of the solutions. Bioxcell®, a commercially available extender used for bull semen was used as a positive control. The diluted semen samples were then chilled at 4oC for 2 hours before evaluation. Next, the samples were packed in 0.25 ml straws and frozen in liquid nitrogen at -196oC for 30 minutes. Frozen semen were thawed at 37oC and manual evaluation for the progressive motility was done. The results indicate treatments with 16% and 20% RPO had greater sperm motility after cryopreservation of 1% (P < 0.05) as compared with the negative control. Treatments with 0%, 4% and 8% RPO showed low pH which may have killed the sperms. The treatment with 16% RPO had the greatest sperm motility among the treated groups for both chilled and frozen-thawed semen. In conclusion, the use of 16% RPO in Tris-based extender enhanced the sperm progressive motility for chilled and frozen-thawed bull semen.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	30 Apr 2024 02:58
Last Modified:	30 Apr 2024 02:58
URI:	http://psaspb.upm.edu.my/id/eprint/1715

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