Molecular detection of mycoplasma spp. and risk factors in dogs from animal shelter in Selangor

Mohd Zahir., Nur Azira (2022) Molecular detection of mycoplasma spp. and risk factors in dogs from animal shelter in Selangor. [Project Paper] (Submitted)

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Abstract

Canine hemotropic mycoplasma is a tick-borne bacterial disease in dogs which most likely to be transmitted by Rhipicephalus sanguineus ticks. Commonly reported hemoplasma, Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum adhere to the surface of erythrocytes of the vertebrate hosts. This disease often causes mild symptoms unless immunocompromised or in splenectomized dogs where infection can lead to severe anemia. This study was aimed to detect the presence of Mycoplasma spp in rescued dogs through conventional Polymerase Chain Reaction (PCR) amplification and microscopic examination of Giemsa-stained thin blood smear. Blood samples were collected via cephalic venipuncture from dogs in four shelters (n = 71) in Selangor. Molecular detection of Mycoplasma spp was performed by amplification of 16SrRNA gene using genus and species-specific primer. Positive samples were sent for sequencing and BLASTsearch in NCBI GenBank for species confirmation. A total of 32 dogs (45.1%) were positive for Mycoplasma haemocanis. According to this study, shelters that are in urban areas and have less exposure to rodents and wild animals are more likely to be infected with Mycoplasma spp (p-value<0.05). However, there is no significant association between tick infestation and mycoplasma infection (p-value = 0.753, χ2 = 0.099) although tick is believed to be the vector for this blood parasite. No significant associations found between intrinsic characteristics of dogs which are sex (χ2 = 0.918, df=1, p-value = 0.338) and age (χ2 = 0.230, df=1, p-value = 0.631). The present study revealed high detection of Mycoplasma haemocanis among rescued dogs from different shelters in Selangor.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	30 Apr 2024 08:05
Last Modified:	30 Apr 2024 08:05
URI:	http://psaspb.upm.edu.my/id/eprint/1738

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