Molecular and serological detection of canine distemper virus in domestic dogs from animal shelters across Klang Valley

Azman, Nur Eizzah (2022) Molecular and serological detection of canine distemper virus in domestic dogs from animal shelters across Klang Valley. [Project Paper] (Submitted)

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Abstract

Canine distemper virus (CDV) is a disease caused by Morbillivirus, a highly contagious RNA virus that attacks the immune system causing severe systemic neurological disease. Chronic form of infection also causes disease known as “hard pad disease”. Dogs that are not vaccinated against CDV, and puppies pose a higher risk of infection. Currently, the prevalence of CDV in Malaysia is still unknown. The goal of this study was to determine the molecular and serological prevalence of CDV in domestic dogs from animal shelters across Klang Valley. A total of five shelters with 10 dogs from each shelter was selected. The serum sample was tested for CDV antibodies with chromatographic immunoassay (CI), while pooled conjunctival and nasal swab samples were tested for RNAs with the use of reverse transcription polymerase chain reaction (RT-PCR) where the hemagglutinin (H) gene was amplified. RT-PCR of pooled conjunctival and nasal swab samples showed 50/50 (100%) negative results indicative of no active infection. The CI results showed 10/50 (20%) negative, 22/50 (44%) high titer, 17/50 (34%) medium titer, and 1/50 (2%) low titer of IgG antibodies against CDV. Most of these dogs had antibodies against CDV which could be due to vaccination history; although the vaccination was not regularly performed. Hence, it is suggested that vaccination protocol employed by these shelters help to reduce disease transmission and risk of infection and provided some degree of herd immunity among the shelter dog population.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	31 May 2024 08:18
Last Modified:	31 May 2024 08:18
URI:	http://psaspb.upm.edu.my/id/eprint/1912

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