Evaluation of mucosal-associated lymphoid tissues (MALTs) in response to post-killed vaccine versus natural infection against streptococcosis (streptococcus agalactiae) in red hybrid tilapia (Oreochromis sp.).

Rusli Lee, Adam Lee (2022) Evaluation of mucosal-associated lymphoid tissues (MALTs) in response to post-killed vaccine versus natural infection against streptococcosis (streptococcus agalactiae) in red hybrid tilapia (Oreochromis sp.). [Project Paper] (Submitted)

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Abstract

Streptococcus agalactiae is one of the most important pathogens of streptococcosis. Streptococcosis affects various aquatic life causing harm to the aquaculture industry worldwide. This study aimed to evaluate the mucosal-associated lymphoid tissues (MALTs) in Red hybrid tilapia (Oreochromis sp.) in response to a post-killed vaccine versus natural infection by immersion methods against S. agalactiae infections. 60 tilapia fingerlings were randomly divided into 4 groups; Group A, Group B, Group C and Group 1. Group A served as a control group. For Group B, fish were immersed once for 1 minute in formalin-killed bacteria (FKB) of S. agalactiae. For Group C, fish were vaccinated once and boosted 1 minute per session one week after the first vaccination. For Group 1, the fish were immersed once for 15 minutes in 2 L of tryptic soy broth (TSB) containing S. agalactiae (109 CFU/mL). The fingerlings were monitored for clinical signs and mortality throughout the study. Five fish from each group were sacrificed one week after their respective immersion and skin and gill samples were taken for histological analysis. Statistical analysis of skin epidermal thickness and gill inflammatory cell area showed that there was no significant difference (p>0.05) in lymphoid tissue in gills mucosal and skin between the groups. Vaccination and natural infection by the immersion method were found to show no significant difference in the development of lymphoid tissue in the gill mucosal and the skin of the fish.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	31 May 2024 08:21
Last Modified:	31 May 2024 08:21
URI:	http://psaspb.upm.edu.my/id/eprint/1914

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