Molecular detection of potentially zoonotic enteric protozoa infecting synanthropic rodents in Selangor And Kuala Lumpur, Malaysia

Zainol, Irdina (2022) Molecular detection of potentially zoonotic enteric protozoa infecting synanthropic rodents in Selangor And Kuala Lumpur, Malaysia. [Project Paper] (Submitted)

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Abstract

Synanthropic rodents are species that live in proximity to humans and serve as reservoirs host for various pathogens. They play an important role in disease transmission to humans and other animal species. One of the concerns affecting worldwide public health is zoonotic parasite diseases. The intricate parasite transmission system permits interactions among humans, animals, vectors, and the environment. For many pathogenic microbial species, synanthropic rodents are also known to serve as both a significant reservoir and an intermediate host. This study was conducted to determine the molecular prevalence of selected rodent-borne enteric protozoa of zoonotic importance, namely Cryptosporidium,Blastocystis and Giardia in various peri domestic areas in Selangor and Kuala Lumpur, Malaysia. Faecal samples were collected from 31 rodents within Selangor and Kuala Lumpur, Malaysia. Samples were taken from rodents of both sexes and from a range of species inhabiting residential areas, commercial areas (wet markets, and shop lots) as well as parks, and forest fringes. Genomic DNA was extracted from faecal samples and polymerase chain reaction (PCR) was done to amplify a partial fragment of the different genes using genus-specific primers to determine the molecular presence of Giardia, Blastocystis, and Cryptosporidium. The highest molecular prevalence (71%) was observed for Cryptosporidium, followed by Giardia (16.1%) and Blastocystis (9.7%). Fisher’s Exact Test did not reveal any significant (p>0.05) gender, species or habitat risk association. The present study has revealed a high prevalence of potentially zoonotic enteric protozoa among the rodents examined. It further contributes to the understanding of the diversity of parasites found in synanthropic rodents, with implications for zoonotic transmission and public health.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	15 Jul 2024 08:13
Last Modified:	15 Jul 2024 08:13
URI:	http://psaspb.upm.edu.my/id/eprint/1963

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