Morphological and molecular identification of trematode cercaria in lymnaeid snails from selected buffalo farms under oil palm intergration in Perak, Malaysia

Puvanarajah, Rhubhithra (2022) Morphological and molecular identification of trematode cercaria in lymnaeid snails from selected buffalo farms under oil palm intergration in Perak, Malaysia. [Project Paper] (Submitted)

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Abstract

Lymnaeid snails play an essential role in the transmission of trematode parasitic flatworms that can infect animals and humans. The snails release trematode cercaria into the environment, encysts into metacercaria which is the infective stage of trematode. There are limited and scarce data available on the trematode cercaria in Malaysia. Therefore, this study is to investigate the morphological and molecular characteristics of trematode cercaria in Lymnaeid snails collected from several buffalo farms under oil palm integration in Perak. A total of 876 Lymnaeid snail samples were collected, and the snails were crushed between two glass slides to observe the trematode cercarial emergence. The identification of trematode cercaria was done by the morphological characteristics of cercaria. Furthermore, the obtained cercaria were identified XII molecularly using polymerase chain reaction (PCR). The infection rate by trematode cercarial emergence in Lymnaeid snails was 8.73% (73/836). Based on morphological characteristics, four types of trematodes cercaria were identified: Echinostome, Xiphidiocercaria, Gymnocephalous and Furcocercous cercaria. Echinostome cercaria showed the highest infection rate in Lymnaeid snails 78.1% (57/73), followed by Xiphidiocercaria 26% (19/73). Both Gymnocephalous cercaria and Furcocercous cercaria had 4.1% (3/73) infection rate. The sequencing analysis of the cercarial internal transcribed spacer 2 (ITS2) showed three cercaria morphotypes belonged to three trematode species: Plagiorchis, Echinostome and Fasciola. These three trematode species belong to digenean trematode that are zoonotic. It is the first report on zoonotic trematode cercaria in Lymnaeid snails in Perak. In conclusion, four trematode cercaria types morphologically and three trematode cercaria species molecularly were identified in the present study. Hence, it is essential to control the snail population in water resources to prevent cercarial infection and further prevent the spread of zoonotic trematode in definitive host.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	29 Jul 2024 02:55
Last Modified:	29 Jul 2024 02:55
URI:	http://psaspb.upm.edu.my/id/eprint/1999

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