Mohd Yusof, Farra Izwanie (2023) Evaluation of phosphanecopper (I) benzoylthiourea series as an alternative antimicrobial treatment for gram-positive bacteria isolated from feline urinary tract infection. [Project Paper] (Submitted)
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Abstract
Antimicrobial resistance (AMR) is undoubtedly one of the greatest challenges of veterinary medicine. AMR incidence is increasing because of the extensive use of antimicrobial drugs in veterinary medicine. In addition, the close interaction between pet animals and humans enables the transmission of AMR bacteria, further contributing to this issue. In small animal practice, urinary tract infections (UTIs) are frequently diagnosed, and often necessitate the use of empirical antibiotics as a treatment approach. Hence, the purpose of this study is to evaluate the antimicrobial effect of phosphanecopper-(I) benzoylthiourea series as an alternative antimicrobial treatment for gram-positive bacteria, specifically Staphylococcus pseudintermedius (S. pseudintermedius) and Enterococcus faecalis (E. faecalis). Two archived gram-positive bacteria were isolated from feline species infected with UTIs from Veterinary Bacteriology Laboratory, University Putra Malaysia. The isolates were collected between 1st January 2021 until 30th September 2022 from feline species of both genders at various ages with different backgrounds. The archived isolates were subcultured on nutrient agar prior to conducting antimicrobial susceptibility tests (AST), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). AST was performed using Kirby-Bauer disk diffusion method and agar well diffusion method on Muller-Hinton (MH) agar plates. Additionally, MIC was determined using broth dilution method, and the MBC was determined on MH agar plates. Selected compounds from phosphanecopper(I) benzoylthiourea series were tested at various concentrations such as Cu(I)ITri (3.125μM, 1.56μM, 0.78μM, 0.39μM); Cu(I)ITriDie (3.125μM, 1.56μM, 0.78μM, 0.39μM); Cu(I)ITriSar (6.25μM, 3.125μM, 1.56μM, 0.78μM) and Cu(I)ITriGly (25μM, 12.5μM, 6.25μM, 3.125μM). AST results revealed that both isolates were not susceptible to any of the selected copper compounds at various tested concentrations, as no clear zone of inhibition was observed in both disk diffusion and agar well diffusion methods. Moreover, the MIC and MBC results also demonstrated that neither of the selected copper compounds was effective against both isolates, as no clear well was visible, and the MBC result was too numerous to count (TNTC). Therefore, Cu(I)ITri, Cu(I)ITriDie, Cu(I)ITriSar and Cu(I)ITriGly from the Phosphanecopper (I) Benzoylthiourea series do not have significant antibacterial activity against archived gram-positive bacteria, specifically S. pseudintermedius and E. faecalis, which were isolated from feline species infected with UTIs. Additional research is necessary, potentially involving a redesign of the ligand utilised in the copper compound.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms Nurhaznita Mahmood |
| Date Deposited: | 15 Jul 2024 08:24 |
| Last Modified: | 15 Jul 2024 08:24 |
| URI: | http://psaspb.upm.edu.my/id/eprint/2015 |
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