Evaluation of ganoderma lucidum as an antioxidant supplement in goat semen cryopreservation

Zulkefli, Aminah Saudah Mazidah (2023) Evaluation of ganoderma lucidum as an antioxidant supplement in goat semen cryopreservation. [Project Paper] (Submitted)

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Abstract

The application of natural antioxidants has gained considerable attention as a potential strategy to enhance the quality and viability of cryopreserved spermatozoa due to its function in protecting the spermatozoa from oxidative injury caused by freezing and thawing. Ganoderma lucidum (GL), a medicinal mushroom with well-documented antioxidant properties, has emerged as a promising candidate for improving the success of semen cryopreservation in various animal species. This study explores the potential of GL as an antioxidant supplement in preserving the quality of buck spermatozoa post-cryopreservation through evaluating the impact of it on sperm viability and motility. Semen samples were collected without pooling from a total of three individual bucks with ages ranging from three to five years at Ladang 15 UPM until ten ejaculates were achieved. The ejaculates were collected using artificial vagina (AV) with the presence of a three years old teaser doe. The neat semen was analysed for its quality, and only semen samples with at least 70% general motility were further processed and being diluted into Tris-egg yolk extenders that contain three different concentrations of GL extracts. The data results were analysed using two-way ANOVA and Kruskal-Wallis H test, showing that sperm that were cryopreserved with 0%, 0.5% and 1.0% GL in semen extender has no significant difference (p>0.05) toward sperm quality parameters including individual progressive movement (%), viability (%) and morphological abnormality (%) at post-thaw. Superoxide dismutase (SOD) data analysis also showed that it is not statistically significant despite having GL as antioxidant supplement. In conclusion, supplementation of GL in the extender is yet to be proven as an excellent choice of antioxidant for goat semen cryopreservation. However, further research against the functions of GL in preserving the sperm quality at post-thaw should be conducted.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	12 Aug 2024 02:45
Last Modified:	12 Aug 2024 02:45
URI:	http://psaspb.upm.edu.my/id/eprint/2032

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