Occurrence of escherichia coli and its antimicrobial resistance in forest birds and environment in Sultan Idris Shah Forestry Education Centre (SISFEC)

Ng, Jia Jie (2023) Occurrence of escherichia coli and its antimicrobial resistance in forest birds and environment in Sultan Idris Shah Forestry Education Centre (SISFEC). [Project Paper] (Submitted)

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Abstract

Escherichia coli is responsible for many pathogenic infections in humans and found in gastrointestinal tracts of warm-blooded animals like birds. Wild birds harbouring E. coli have the potential to transmit disease and increase spread of antimicrobial resistance due to their high mobility. Therefore, the objective of this study is to determine the occurrence of E. coli and the antimicrobial resistance from the E. coli colonies isolated from the cloacal swab sample of wild forest birds and environmental swab samples in Sultan Idris Shah Forestry Education Centre (SISFEC) located in Ayer Hitam Forest Reserve, Puchong, Selangor, Malaysia. A total of eight wild forest birds of various species were caught with mist-nets and cloacal swab samples were taken from each bird, while seven environmental swab samples were taken from the forest floor, streams and faecal samples associated with the congregation or presence of birds. The swab samples were selectively cultured, isolated, and confirmed of E. coli occurrence by biochemical tests, before being subjected to antimicrobial susceptibility tests (AST). The study results showed that two (25%, 2/8) cloacal samples and three (42.9%, 3/7) environmental samples were positive for E. coli. Amongst the eighteen E. coli isolates subjected for AST, four isolates (22.2%, 4/18) from a female adult Oriental Magpie Robin (Copsychus saularis) showed multidrug-resistance towards ampicillin, trimethoprim, and tetracycline, while one isolate (5.6%, 1/18) from the environmental samples of bird droppings on a tree leaf showed multidrug-resistance towards ampicillin, aztreonam, cephalexin, and nalidixic acid. The most frequent resistance observed for E. coli isolated was towards ampicillin (27.8%, 5/18), followed by trimethoprim and tetracycline (22.2%, 4/18), and least to aztreonam, cephalexin, and nalidixic acid (5.6%, 1/18). In conclusion, wild forest birds and environments associated with birds in SISFEC can be a source of multidrug-resistant E. coli, thus it is important to be vigilant in biosafety measures during handling of wild forest birds, and more studies on the antimicrobial resistance profile in wild birds in the same and adjacent regions can be conducted.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	12 Aug 2024 02:53
Last Modified:	12 Aug 2024 02:53
URI:	http://psaspb.upm.edu.my/id/eprint/2034

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