Molecular detection of Mycoplasma spp. in pet dogs from Johor Bahru, Malaysia

Mohd Kamal, Fatin Nadia (2023) Molecular detection of Mycoplasma spp. in pet dogs from Johor Bahru, Malaysia. [Project Paper] (Submitted)

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Abstract

Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum are two common species of hemotropic Mycoplasma with poorly understood transmission routes. Rhipicephalus linnaei previously known as Rhipicephalus sanguineus has been suggested as a potential tick vector and transmission could also occur through fomites, aggressive interaction, blood transfusion, and vertical transmission. Despite the large population of pet dogs in Malaysia, there is a scarce of information on detection of Mycoplasma spp in pet dogs in the country. Generally, dogs infected with hemotropic Mycoplasma remain asymptomatic unless immunocompromised or splenectomised which could lead to hemolytic anaemia. This study aimed to detect the presence of Mycoplasma spp. in pet dogs from Johor Bahru, providing valuable insights into the frequency of these pathogens. 169 canine blood samples from Pet Expo events by private clinic from Johor Bahru were sent to the Parasitology Laboratory in the Veterinary Laboratory Service Unit, Universiti Putra Malaysia (VLSU-UPM) for blood parasite detection. A conventional Polymerase Chain Reaction (PCR) was used to determine the presence of hemotropic Mycoplasma by amplification of 16S rRNA using genus and species-specific primers. The positive DNA products were sequenced and identified at the species level for confirmation. Overall, the molecular detection of Mycoplasma spp. in pet dogs from Johor Bahru, Malaysia was 11.8% (n=20/169), which comprised 95% (n=19/20) for Mycoplasma haemocanis and 5% (n=1/20) for Candidatus Mycoplasma haematoparvum. The significant association between demographic information and mycoplasmosis was unable to be demonstrated due to limited records from the clinic. However, demographic patterns for positive dogs in this study revealed male, adult and purebreds had a greater risk of infection. The majority of them were kept indoors and not dewormed. In conclusion, the present study revealed significant detection of Mycoplasma haemocanis and low detection of Candidatus Mycoplasma haematoparvum among pet dogs from Johor Bahru. Thus, the infected dogs should be given treatment and ectoparasiticide as recommended by the veterinarian accordingly.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	12 Aug 2024 03:00
Last Modified:	12 Aug 2024 03:00
URI:	http://psaspb.upm.edu.my/id/eprint/2035

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