Ab Wahid, Muhammad Azriuddin (2017) Isolation and characterisation of microplastics in whitecheek shark (Carcharhinus dussumieri) and pale-edged stingray (Dasyatis zugei) collected from Malaysia water. [Project Paper] (Submitted)
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Abstract
Introduction: Nearly 150 million tons of plastic debris present in the ocean and are estimated to increase in the future. Microplastics were found in various marine organisms such as zooplankton, mussels, clams and fish. There were many research regarding ingestion of microplastics in fish but no study was done on Malaysian marine vertebrate organisms. Objectives: To compare microplastics (MPs) loads between whitecheek shark ( Carcharhinus dussumieri) and pale-edged stingray (Dasyatis zugei). Methodology: Fish dissection conducted to isolated internal organ. Intestinal organ for each sample were digested in 10% potassium hydroxide (KOH). The digested solution then vacuum filtered using 149 µm filter paper. The filter paper had been soaks with sodium iodide and centrifuge for density separation. Then, the supernatant filtered using 8 µm filter paper and being observed using microscope to find MPs and determine it morphology. For characterization steps, micro-Raman microscopy used to identify plastics polymer composition and additives composition. Results and Discussion: Only an additive particle (phthalocyanine) found in this study. There were no significant different in all parameters (plastic polymer, morphology and additives) between two species. A factor possible no MPs found in this study due to Carcharhinus dussumieri and Dasyatis zugei had excreted MPs or due it feeding behaviour. Another possible factor is the technical limitation because 149µm filter membrane used in filtering the digested intestinal material. There were possible loss MPs sized less than 149 µm. There also possibility of no MPs pollution at study area. Conclusion: For now, C. dussumieri and D. zugei from Endau-Rompin coastal area not polluted with microplastics. So, the C. dussumieri and D. zugei from Malaysian water probably safe to be consumed by human or to use in other byproducts. Keywords: Microplastics, Isolation, Shark, Ray, Raman Spectroscopy.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms Norafizah Radzuan |
| Date Deposited: | 24 Oct 2024 06:45 |
| Last Modified: | 24 Oct 2024 06:45 |
| URI: | http://psaspb.upm.edu.my/id/eprint/2054 |
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