Occurrence of avian pathogenic escherichia coli (APEC) in commercial broiler chicken with chronic complicated respiratory disease (CCRD)

Huzairi, Nur Aimi Syifa (2023) Occurrence of avian pathogenic escherichia coli (APEC) in commercial broiler chicken with chronic complicated respiratory disease (CCRD). [Project Paper] (Submitted)

Text lp fpv 2023 29.pdf Download (1MB)

Abstract

Avian colibacillosis is commonly associated with complicated chronic respiratory disease (CCRD) in chickens caused by Avian Pathogenic Escherichia coli (APEC). It is reported as the major bacterial disease in the poultry industry that leads to a considerably large economic loss. The purpose of this study was to determine the occurrence of APEC from chickens that were showing signs of CCRD such as swollen head, ruffled feathers and stunted growth. A total of 30 cloacal swabs samples chickens were collected from a broiler farm in Selangor. Samples were initially inoculated onto MacConkey agar and nutrient agar to isolate presumptive E. coli cultures. Subsequently, the identification of E. coli was done using a series of biochemical test which include oxidase test, triple sugar iron test, sulphide-indole-motility (SIM) test, urea test and citrate test. Next, multiplex polymerase chain reaction (PCR) was used to identify APEC from the E. coli isolates obtained. A total of eight primer sets namely astA, iss, irp2, papC, iucD, tsh, vat and cva/cvi were used to determine the presence of virulent genes in the isolates. Isolates possessing four or more virulent genes are considered as APEC. The antibiotic susceptibility profiles for the APEC isolates were conducted using the standard Kirby Bauer disc diffusion technique. The results of the multiplex polymerase chain reaction showed that there were various distribution of virulence genes amongst the isolates. Thirteen out of 42 E. coli (31%) isolates were identified as APEC. All APEC isolates contain the iucD virulent gene. The second highest prevalent gene detected were iss and irp2. In contrast, astA showed the least prevalent gene as it was only found in 1 out of 13 APEC isolates that was identified. APEC isolates were found to be 100% resistant towards ampicillin and tetracycline. It also showed 77% resistant towards amoxicillin and high intermediate of 69% for enrofloxacin. For gentamicin and chloramphenicol, the isolates showed susceptibility of 85% and 77% respectively. In conclusion, there were presence of APEC in chickens presenting CCRD signs with iucD, an iron acquisition system gene as it most prevalent gene. The APEC isolates can be considered as multidrug resistant bacteria.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	30 Sep 2024 06:58
Last Modified:	30 Sep 2024 06:58
URI:	http://psaspb.upm.edu.my/id/eprint/2073

Actions (login required)

View Item