Histological and histomorphometrical assessments of feline osteoarthritis (OA) menisci

Sukry, Nur Salina (2023) Histological and histomorphometrical assessments of feline osteoarthritis (OA) menisci. [Project Paper] (Submitted)

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Abstract

Feline osteoarthritis (OA) is a chronic degenerative process affecting feline synovial joints. Menisci are one of the components of synovial joints that are located between femoral condyles and the tibial plateau. It is a fibrocartilaginous structure found in pairs laterally and medially, responsible for load distribution, shock absorption, stabilisation and joint lubrication. Understanding meniscal anatomy is a prerequisite, as it has a significant role in the initiation and progression of stifle OA. The current study aimed to evaluate the histological and histomorphometrical changes in feline OA menisci. Besides, this study focuses on determining the association between meniscal mineralisation and degeneration in feline OA. A total of thirty-eight archived paraffin-embedded skeletally mature cat cadavers' meniscal tissues, with (n=15), without naturally occurring OA (n=8) and OA with meniscal mineralisation (MM) (n=15), were used. The archived paraffin-embedded tissues were sectioned at 5.0μm using a microtome and mounted onto glass slides. The longitudinal cross sections were stained using haematoxylin and eosin (H&E) and Safranin O for meniscal evaluation. The histologic assessment was evaluated microscopically and scored using a histological grading system based on parameters such as surface integrity, collagen organisation and safranin O intensity. In addition, histomorphometric evaluations were performed using a light microscope at X4 and X10 magnifications connected to a computer equipped with Motic Image Devices software. The parameters assessed were cellular density (cell/mm2) and the size of MM (mm2). Histological analysis revealed that the global score of OA menisci was significantly higher (P<0.05) than the normal menisci. There was no significant difference in cell density between normal and OA menisci, but opposite results were found between OA (without MM) and OA (with MM). There was also a significant moderate correlation (rS=0.6712) between the MM area and OA (with MM) global score. The current study revealed remarkable changes in feline OA in histological and histomorphometrical parameters.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	30 Sep 2024 07:30
Last Modified:	30 Sep 2024 07:30
URI:	http://psaspb.upm.edu.my/id/eprint/2078

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