Lipid peroxidation and catalase activity in African catfish (Clarias gariepinus): the effect of exposure to butachlor

Wan Suhaimi, Wan Muhamad Izzat Farhan (2016) Lipid peroxidation and catalase activity in African catfish (Clarias gariepinus): the effect of exposure to butachlor. [Project Paper]

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Abstract

Background: The widespread use of chemicals to control pest weeds has been recognized in agricultural practices. Indiscriminate use of these chemicals to improve agricultural production and yield may result in aquatic pollution due to rain and surface runoff. Butachlor (BUC), 2-chloro-N (2, 6-diethylphenyl) acetamide is an herbicide that is widely used to control perennial grasses and some broad leaf weeds in Asia, South America and Africa. Two biomarkers lipid peroxidation (LPO) and catalase (CAT) activity) were adapted for application to African catfish (Clarias gariepinus) to see the effect on level of LPO product and CAT activity after BUC-exposed. Objective: To determine the health effects of herbicides posed on human using a vertebrate models Clarias gariepinus. Method: Juvenile Clarias gariepinlts had been exposed to three different concentrations (50 ug/L, 100 ug/L and 150 ug/L) BUC for 21 days. Meanwhile, the non-exposed group was considered as control group. After the exposure, fish was killed and liver was sampled and stored at -80 C. Lipid peroxidation (LPO) level and CAT activity was measured in the liver using enzyme-linked immunsorbent assay (ELISA) method. Result: The results obtained showed there is significant different (p<0.05) between exposed group with the control group for the LPO level. CAT also showed a significant decrease between control and exposed groups. Conclusion: From the present study it is concluded that BUC is harmful to the fish and probably human health. The application of BUC in paddy fields may put the health of farmers at risk. Keywords: butachlor, biomarkers, catalase, lipid peroxidation, African catfish (Clarias gariepinus)

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	28 Oct 2024 01:50
Last Modified:	28 Oct 2024 01:50
URI:	http://psaspb.upm.edu.my/id/eprint/2101

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